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Emilio Lamazares
Researcher at University of Concepción
Publications - 11
Citations - 196
Emilio Lamazares is an academic researcher from University of Concepción. The author has contributed to research in topics: Pichia pastoris & Thermolysin. The author has an hindex of 5, co-authored 10 publications receiving 164 citations. Previous affiliations of Emilio Lamazares include University of Zaragoza.
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Journal ArticleDOI
Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris.
Manuel Mansur,Cecilia Cabello,Lester Hernández,José M. Pais,Laura Varas,Jorge Valdés,Yanet Terrero,Abdel Hidalgo,Liuba Plana,Vladimir Besada,Liudys García,Emilio Lamazares,Lila Castellanos,Eduardo Martínez +13 more
TL;DR: A direct relationship between protein production in Pichia pastoris and the number of introduced synthetic genes of miniproinsulin (MPI), fused to the Saccharomyces cerevisiae pre-pro alpha factor used as secretion signal, and inserted between the alcohol oxidase 1 promoter and terminator sequences is found.
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Rational stabilization of complex proteins: a divide and combine approach
TL;DR: It is shown that the key to stabilize the thermostability of proteins is to know the regions of lower stability, and engineered apoflavodoxin, a non-fully cooperative protein on which previous thermostabilizing attempts had failed.
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Kinetic study of removal heavy metal from aqueous solution using the synthetic aluminum silicate.
Manuel A. Treto-Suárez,Julio Omar Prieto-García,Ángel Mollineda-Trujillo,Emilio Lamazares,Yoan Hidalgo-Rosa,Karel Mena-Ulecia +5 more
TL;DR: Absorption kinetics studies of heavy metals in aqueous solutions, through more widespread models, have demonstrated that A l 2 O 3 3 S i O 2 H 2 O has excellent properties as absorbents of this material.
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Recombinant expression of ShPI‐1A, a non‐specific BPTI‐Kunitz‐type inhibitor, and its protection effect on proteolytic degradation of recombinant human miniproinsulin expressed in Pichia pastoris
Dayrom F. Gil,Rossana García-Fernández,Maday Alonso-del-Rivero,Emilio Lamazares,Mariela Pérez,Laura Varas,Joaquín Díaz,María A. Chávez,Yamile González-González,Manuel Mansur +9 more
TL;DR: A broad specificity protease inhibitor such as rShPI-1A can be used to improve the yield of recombinant proteins secreted from P. pastoris and protect a model protein, recombinant human miniproinsulin (rhMPI), from proteolytic degradation during expression.
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Post-translational modification of a chimeric EPO-Fc hormone is more important than its molecular size in defining its in vivo hematopoietic activity.
Emilio R. Salgado,Raquel Montesino,Sivana P. Jiménez,Mauricio Ostria González,Florence Hugues,Oscar I. Cabezas,Rafael Maura-Perez,Paulina Saavedra,Emilio Lamazares,Alexis Salas-Burgos,Juan Carlos Vera,Oliberto Sánchez,Jorge R. Toledo +12 more
TL;DR: The low content of tri- or tetra-antennary sialylated N-glycans linked to the chimeric EPO-Fc hormone, expressed in the goat mammary gland epithelial cells, defined its in vivo hematopoietic activity.