Showing papers by "Eric J. Jenkinson published in 2007"
TL;DR: A specialized role for PDGFRalpha+ fetal mesenchyme in the thymus is highlighted by determining availability of thymic niches through the regulation ofThymic epithelial proliferation through theregulation of platelet-derived growth factor receptor alpha expression.
105 citations
TL;DR: An ontogenetic program of lymph node stromal cell maturation is defined in relation to the requirement for LTic's and analysis of the lymph node anlagen in normal and lymphotoxin a (LTa)-deficient embryos shows that LTa-mediated signaling is required to sustain proliferation and survival ofStromal cells in vivo.
67 citations
TL;DR: Modifications of the basic fetal thymus organ culture system, such as hanging drop cultures and reaggregate thymUS organ cultures, provide useful systems to analyze thymu colonization and thymic stromal cell function, respectively.
Abstract: INTRODUCTIONThe generation of functionally competent T-cells from their progenitors involves a series of developmental events including proliferation, differentiation, and survival. T-cell development is a non-cell-autonomous event, and requires interactions with thymic stromal cells. Fetal thymus organ cultures provide an in vitro system in which isolated embryonic thymus lobes can be maintained in culture, allowing the study of T-cell development as well as thymic stromal cell function. This system remains the only in vitro system that supports a complete program of T-cell development, including positive and negative selection of the developing T-cell receptor repertoire. Modifications of the basic fetal thymus organ culture system, such as hanging drop cultures and reaggregate thymus organ cultures, provide useful systems to analyze thymus colonization and thymic stromal cell function, respectively.
36 citations
Journal Article•
TL;DR: A specialized role for PDGFRα + fetal mesenchyme in the thymus is highlighted by determining availability of thymic niches through the regulation ofThymic epithelial proliferation via platelet-derived growth factor receptor a (PDGFRa) expression.
14 citations
TL;DR: In their study, Rossi et al. show that, in contrast to previous reports, Mts24 is not a progenitor marker for embryonic epithelial cells capable of creating the required thymic microenvironment for T cell development.
Abstract: The immunohistochemical analysis of a frozen section of a thymus graft is taken from the article by Rossi et al. (pp. 2411–2418). The image shows cytokeratin expression in green and Aire expression (medullary epithelial cells) in red. The graft shown is generated from the EpCAM1+Mts24+ thymic epithelial subset obtained at embryonic day 16 (BALB/c mice). In their study, Rossi et al. show that, in contrast to previous reports, Mts24 is not a progenitor marker for embryonic epithelial cells capable of creating the required thymic microenvironment for T cell development.
1 citations