Eric M. Brustad

TL;DR: E engineered variants of cytochrome P450BM3 that catalyze highly diastereo- and enantioselective cyclopropanation of styrenes from diazoester reagents via putative carbene transfer are reported, highlighting the capacity to adapt existing enzymes for the catalysis of synthetically important reactions not previously observed in nature.

TL;DR: A unique serine-heme ligated cytochrome “P411” that catalyzes efficient and selective carbene transfers from diazoesters to olefins in intact Escherichia coli cells is designed.

TL;DR: A marriage of ingenuity and evolution will expand the scope of protein function well beyond Mother Nature's designs.

TL;DR: In this article, an orthogonal labeling strategy was proposed to make SDSL amenable to any protein, i.e., one that does not rely on any of the functional groups found in the common 20 amino acids.

TL;DR: A genetically encoded unnatural ketone amino acid was labeled with a hydroxylamine-containing fluorophore with high yield and specificity, allowing the study of T4 lysozyme folding at single-molecule resolution.