Erica Elisa Ferrandi

TL;DR: The hydroxysteroid dehydrogenases (HSDHs)-catalyzed one-pot enzymatic synthesis of 12-ketoursodeoxycholic acid (3a,7b-dihydroxy-12-oxo-5b-cholanoic acid), a key intermediate for the synthesis of ursodeoxyCholic acid, from cholic acid has been investigated.

TL;DR: An update about the availability of microbial ATAs, discovered by both screening and database-mining approaches, or obtained by protein engineering of wild-type enzymes, will be provided, and innovative biocatalytic process strategies aimed at the improvement of ATAs performances in chiral amines synthesis will be presented.

TL;DR: Applications of the so-called laccase-mediator system (LMS) for alcohol oxidation are discussed with a focus on carbohydrate chemistry and natural products modification as well as on bio- and chemo-integrated processes.

TL;DR: Both enzymes showed a very strong substrate inhibition with all the tested substrates, whereas ursocholic acid was the most effective inhibitor of 7β-HSDH activity.

TL;DR: Functional characterization of the novel ATAs discovered in the metagenome of samples collected from hot springs in Iceland and in Italy demonstrated that they all possess a thermophilic character and are capable of performing amine transfer reactions using a broad range of donor and acceptor substrates, suggesting a good potential for practical synthetic applications.