E
Eriko Takano
Researcher at University of Manchester
Publications - 149
Citations - 13383
Eriko Takano is an academic researcher from University of Manchester. The author has contributed to research in topics: Streptomyces coelicolor & Synthetic biology. The author has an hindex of 46, co-authored 145 publications receiving 11472 citations. Previous affiliations of Eriko Takano include Norwich Research Park & Engineering and Physical Sciences Research Council.
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Construction of thiostrepton-inducible, high-copy-number expression vectors for use in Streptomyces spp.
TL;DR: A high-copy-number plasmid expression vector that contains a thiostrepton-inducible promoter, PtipA, from Streptomyces lividans 66 and a derivative of pIJ6021, pIJ4123, can be used to produce His-tagged fusion proteins that can be readily purified by Ni(2+)-affinity chromatography.
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Deletion of a regulatory gene within the cpk gene cluster reveals novel antibacterial activity in Streptomyces coelicolor A3(2).
TL;DR: It is suggested that scbR2 may act in a negative feedback mechanism to eventually limit yCPK biosynthesis, and the absence of the newly described compounds from cpk deletion mutants, suggest that they are products of the previously orphan cpk biosynthetic pathway in which abCPK is converted into the yellow pigment.
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A systematic computational analysis of biosynthetic gene cluster evolution: lessons for engineering biosynthesis.
Marnix H. Medema,Peter Cimermancic,Andrej Sali,Eriko Takano,Michael A. Fischbach,Michael A. Fischbach +5 more
TL;DR: In this article, the authors perform a systematic computational analysis of BGC evolution and derive evidence for three findings that shed light on the ways in which nature successfully invents new molecules: 1) BGCs for complex molecules often evolve through the successive merger of smaller sub-clusters, which function as independent evolutionary entities.
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A rare leucine codon in adpA is implicated in the morphological defect of bldA mutants of Streptomyces coelicolor.
Eriko Takano,M. Tao,M. Tao,Feng Long,Maureen J. Bibb,Lei Wang,W. Li,Mark J. Buttner,Mervyn J. Bibb,Deng Zixin,Deng Zixin,Keith F. Chater +11 more
TL;DR: AdpAc, an S. coelicolor gene similar to the Streptomyces griseus A factor‐regulated adpAg, was found to complement the bldH109 mutant partially at both single and multiple copies, and revealed that translational arrest at the UUA codon in adpAc mRNA is the principal target through which bldA influences morphological differentiation.
A Systematic Computational Analysis of Biosynthetic Gene Cluster Evolution: Lessons for Engineering Biosynthesis - eScholarship
Andrej Sali,Michael A. Fischbach,Marnix H. Medema,Peter Cimermancic,Eriko Takano,MA Fischbach +5 more
TL;DR: By performing a systematic computational analysis of BGC evolution, this work derives evidence for three findings that shed light on the ways in which, despite these constraints, nature successfully invents new molecules.