The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.

SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)

Solid waste management is the one thing just about every city government provides for its residents. While service levels, environmental impacts and costs vary dramatically, solid waste management is arguably the most important municipal service and serves as a prerequisite for other municipal action. As the world hurtles toward its urban future, the amount of municipal solid waste (MSW), one of the most important by-products of an urban lifestyle, is growing even faster than the rate of urbanization. Ten years ago there were 2.9 billion urban residents who generated about 0.64 kg of MSW per person per day (0.68 billion tonnes per year). This report estimates that today these amounts have increased to about 3 billion residents generating 1.2 kg per person per day (1.3 billion tonnes per year). By 2025 this will likely increase to 4.3 billion urban residents generating about 1.42 kg/capita/day of municipal solid waste (2.2 billion tonnes per year).

What a Waste : A Global Review of Solid Waste Management

Biological degradation of plastics: a comprehensive review.

https://scholarlypublications.universiteitleiden.nl/access/item%3A2978611/view

Microplastics in freshwater and terrestrial environments: Evaluating the current understanding to identify the knowledge gaps and future research priorities.

https://bura.brunel.ac.uk/bitstream/2438/18351/1/FullText.pdf

An overview of chemical additives present in plastics: Migration, release, fate and environmental impact during their use, disposal and recycling.

With more and more plastics being employed in human lives and increasing pressure being placed on capacities available for plastic waste disposal, the need for biodegradable plastics and biodegradation of plastic wastes has assumed increasing importance in the last few years. This review looks at the technological advancement made in the development of more easily biodegradable plastics and the biodegradation of conventional plastics by microorganisms. Additives, such as pro-oxidants and starch, are applied in synthetic materials to modify and make plastics biodegradable. Recent research has shown that thermoplastics derived from polyolefins, traditionally considered resistant to biodegradation in ambient environment, are biodegraded following photo-degradation and chemical degradation. Thermoset plastics, such as aliphatic polyester and polyester polyurethane, are easily attacked by microorganisms directly because of the potential hydrolytic cleavage of ester or urethane bonds in their structures. Some microorganisms have been isolated to utilize polyurethane as a sole source of carbon and nitrogen source. Aliphatic-aromatic copolyesters have active commercial applications because of their good mechanical properties and biodegradability. Reviewing published and ongoing studies on plastic biodegradation, this paper attempts to make conclusions on potentially viable methods to reduce impacts of plastic waste on the environment.

A Review of Plastic Waste Biodegradation

http://www.eng.uwo.ca/grc/pdfs/2011/Yanful_arsenic_chromium.pdf

Arsenic and chromium removal by mixed magnetite-maghemite nanoparticles and the effect of phosphate on removal.

Comparison of municipal solid waste management systems in Canada and Ghana: a case study of the cities of London, Ontario, and Kumasi, Ghana.

Chemical states in XPS and Raman analysis during removal of Cr(VI) from contaminated water by mixed maghemite-magnetite nanoparticles.

The soil-water characteristic curve (SWCC) of fine-grained soils is usually determined experimentally. In many applications, such as design of mine waste covers and landfill liners, the unsaturated permeability function, k(h), is often derived theoretically from the measured SWCC. Implicit in these derivations is the transformation of the SWCC to a pore-size distribution (PSD), typically assumed to be constant and mono-modal. PSDs of a clayey till compacted at various water contents were measured after compaction, after flexible-wall permeability testing, and during and after SWCC tests. The measurements show that the PSD changes significantly during permeability and SWCC testing. A method is advanced for predicting the observed changes in PSD during SWCC testing. PSDs are determined for soil samples subjected to the highest and lowest suctions applied during the SWCC test. The measured PSDs are transformed to account for pore trapping; the transform assumes that flow occurs through two sets of randomly d...

Ernest K. Yanful

Papers

A Review of Plastic Waste Biodegradation

Arsenic and chromium removal by mixed magnetite-maghemite nanoparticles and the effect of phosphate on removal.

Comparison of municipal solid waste management systems in Canada and Ghana: a case study of the cities of London, Ontario, and Kumasi, Ghana.

Chemical states in XPS and Raman analysis during removal of Cr(VI) from contaminated water by mixed maghemite-magnetite nanoparticles.

Measurement and estimation of pore shrinkage and pore distribution in a clayey till during soil-water characteristic curve tests