F
F A Lewis
Researcher at University of Leeds
Publications - 38
Citations - 2610
F A Lewis is an academic researcher from University of Leeds. The author has contributed to research in topics: Phosphatase complex & Polymerase chain reaction. The author has an hindex of 23, co-authored 38 publications receiving 2582 citations. Previous affiliations of F A Lewis include Leeds General Infirmary.
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Journal ArticleDOI
Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction.
TL;DR: Several DNA extraction techniques were quantitatively and qualitatively compared using both fresh and paraffin wax embedded tissue and their suitability investigated for providing DNA and RNA for the polymerase chain reaction (PCR).
Journal ArticleDOI
Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.
S. A. Ho,J. A. Hoyle,F A Lewis,A D Secker,D Cross,N P Mapstone,Michael F. Dixon,J. I. Wyatt,D S Tompkins,Graham R. Taylor +9 more
TL;DR: The results of PCR amplification and partial 16S rRNA gene sequence analysis strongly support the contention that the gastric organisms previously recovered from a pig, a baboon, and rhesus monkeys are H. pylori.
Journal ArticleDOI
Prognostic value of p53 overexpression and c-Ki-ras gene mutations in colorectal cancer
Sandra M. Bell,Sandra M. Bell,Nigel Scott,Nigel Scott,D Cross,D Cross,Peter M. Sagar,Peter M. Sagar,F A Lewis,F A Lewis,G. Eric Blair,G. Eric Blair,Graham R. Taylor,Graham R. Taylor,Michael F. Dixon,Michael F. Dixon,Philip Quirke,Philip Quirke +17 more
TL;DR: Screening for multiple genetic abnormalities in colorectal cancers excised at surgery may prove to be a useful tool in determining prognosis.
Journal ArticleDOI
Identification of Helicobacter pylori DNA in the mouths and stomachs of patients with gastritis using PCR.
N P Mapstone,D. A. F. Lynch,F A Lewis,Anthony T. R. Axon,D S Tompkins,Michael F. Dixon,Philip Quirke +6 more
TL;DR: The demonstration of the organism in the mouths of a substantial proportion of dyspeptic patients has major implications for the spread of H pylori and identifies a potential source for reinfection following eradication of the organisms from the stomach.
Journal ArticleDOI
Rapid detection of allele loss in colorectal tumours using microsatellites and fluorescent DNA technology.
TL;DR: A rapid technique which overcomes most of the problems associated with radioactive Restriction Fragment Length Polymorphism (RFLP) analysis of allele loss is developed and analysed 26 normal tissue: cancer pairs for allele loss at two loci linked to the adenomatous polyposis coli (APC) gene on chromosome 5q.