[54] Thin-layer chromatography of lipids

Separations of lipids by silver ion chromatography.

A method has been developed for the analysis of isomeric monoglycerides by thin-layer adsorption chromatography Isomeric monoglycerides are separated on silica gel impregnated with boric acid; a mixed solvent system is used The interaction of hydroxy compounds with boric acid allows the separation of 1- and 2-monoglycerides which cannot be resolved on silica gel alone In order to make the method quantitative, the resolved components are charred and their spot densities measured Glycerol, fatty acids, diglycerides, and triglycerides do not interfere The method does not induce isomerization

Quantitative estimation of isomeric monoglycerides by thin-layer chromatography

Photodensitometry in the thin-layer chromatographic analysis of neutral lipids

Silver ion chromatography of lipids and fatty acids.

The paper describes a procedure involving thin layer chromatography on silica impregnated with silver nitrate, which makes possible the separation of synthetic and natural glyceride mixtures into classes according to their degree of unsaturation, and within those classes the resolution of certain isomeric unsaturated glycerides. The separations depend upon the ability of compounds having an olefinic double bond to form co-ordination complexes with the silver ion.

F. B. Padley

Papers

The quantitative analysis of triglyceride mixtures by thin layer chromatography on silica impregnated with silver nitrate