Fabian Langenbach

TL;DR: The effects of dexamethasone on intracellular signaling cascades that lead to osteogenic differentiation of bone marrow stroma-derived stem cells are described and it is concluded that Dex induces Runx2 expression by FHL2/β-catenin-mediated transcriptional activation and that Dex enhances Runx 2 activity by upregulation of TAZ and MKP1.

TL;DR: This protocol describes an effective method for the production of spherical microtissues (microspheres) using unrestricted somatic stem cells from human umbilical cord blood and adapted protocols for the use of these microspheres in histological analysis.

TL;DR: Cord blood stem cells in combination with ICBM-induced ectopic bone formation in vivo are stronger than ESCs and are considered as statistically significant compared to the basic value.

TL;DR: Microtissue cultures have closer characteristics with cells in vivo and their enhanced osteogenic differentiation makes scaffold-free microtissues a promising concept in osteogenic tissue engineering.

TL;DR: A scaffold-free tissue construct (microspheres) is demonstrated that, if osteogenic differentiated, mineralizes while maintaining the capability to let cells grow out of the united cell structure and it is suggested that cells would migrate also in vivo.