Karin Berr

TL;DR: This protocol describes an effective method for the production of spherical microtissues (microspheres) using unrestricted somatic stem cells from human umbilical cord blood and adapted protocols for the use of these microspheres in histological analysis.

TL;DR: Cord blood stem cells in combination with ICBM-induced ectopic bone formation in vivo are stronger than ESCs and are considered as statistically significant compared to the basic value.

TL;DR: Microtissue cultures have closer characteristics with cells in vivo and their enhanced osteogenic differentiation makes scaffold-free microtissues a promising concept in osteogenic tissue engineering.

TL;DR: A scaffold-free tissue construct (microspheres) is demonstrated that, if osteogenic differentiated, mineralizes while maintaining the capability to let cells grow out of the united cell structure and it is suggested that cells would migrate also in vivo.

TL;DR: DAG treatment can be recommended for generating ESC populations with osteogenic differentiation that are intended for use in bone tissue engineering and appears to be more potent than stimulation with BMP-2 alone.