Showing papers by "Fabien Zoulim published in 1995"
TL;DR: The link between HCV lymphotropism and both the natural history of the viral infection and the immunological disorders frequently observed in HCV infections still needs to be established.
Abstract: The mechanisms of viral persistence are complex and include infection of the lymphoid cells. In the case of hepatitis B virus, early observations have suggested that HBV may infect peripheral blood mononuclear cells (PBMC). In animal modes studies in chronic hepatitis B patients have further confirmed that viral DNA replicative intermediates, as well as viral transcripts and proteins, can be detected in PBMC under certain conditions. The consequences of this lymphotropism are not fully understood, but it seems likely that PBMC represent an extrahepatic reservoir of virus. The ability of hepatitis C virus to infect PBMC has been demonstrated in vivo and in vitro. The link between HCV lymphotropism and both the natural history of the viral infection and the immunological disorders frequently observed in HCV infections still needs to be established. In both cases, the infection of PBMC by HBV or HCV may represent the source of infection of the liver graft in patients transplanted for end-stage liver disease associated with HBV or HCV.
32 citations
4 citations
Journal Article•
TL;DR: This study confirms that pre-S1 antigen detection is a reliable marker of hepatitis B virus replication which can be easily performed in chronically infected patients and is especially useful in identifying anti-HBe positive carriers who replicate a minus pre-core mutant and could benefit from antiviral therapy.
Abstract: Objectif et methodes. - Nous avons etudie de facon prospective la signification clinique et virologique de la detection des antigenes pre-S dans le serum de malades atteints d'infection chronique par le virus de l'hepatite B. Quatre cent trente-sept echantillons de serum successifs, provenant de 116 malades, ont ete testes pour l'antigene pre-S1 et l'antigene pre-S2 a l'aide d'une methode radio-immunologique utilisant des anticorps monoclonaux specifiques. Nous avons determine le rapport antigene pre-S/antigene HBs qui permet d'estimer le nombre d'epitopes pre-S exprimes a la surface des particules virales circulantes. Resultats. - Le rapport antigene pre-S1/antigene HBs etait correle de facon positive avec l'intensite de la replication virale evaluee a l'aide du dosage de l'ADN polymerase virale (P < 0,05). Le rapport antigene pre-S1/antigene HBs etait le plus eleve chez les malades atteints d'hepatite chronique en phase de replication virale active (24% ± 13) ; chez les malades anti-HBe positifs, le rapport antigene pre-S1/antigene HBs etait plus eleve chez les malades repliquant un variant antigene HBe negatif du virus B et atteints d'hepatite chronique (17 % ± 9) que chez les porteurs asymptomatiques de l'antigene HBs (5 % ± 6) (P < 0,05). Le rapport antigene pre-S2/antigene HBs n'etait pas correle avec l'intensite de la replication virale ni avec la situation clinique des malades. Conclusion. - Cette etude confirme que l'antigene pre-S1 represente un marqueur de replication virale, de detection aisee chez les malades atteints d'infection chronique par le virus B. Ce test permet aussi de depister, chez les malades positifs pour l'anticorps anti-HBe, ceux qui repliquent un virus B mutant pouvant eventuellement beneficier d'un traitement par interferon.
3 citations