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Showing papers by "Fernando Ferreira published in 2001"


Journal ArticleDOI
TL;DR: From December 1998 to March 1999, 40 stud farms were studied in the state of São Paulo, Brazil, indicating that mowing all the pastures at least once a year can be considered a protective factor against the presence of mixed overgrowth pastures on the farm, and consequently, againstThe presence of A. cajennense on the horses.

146 citations


Journal ArticleDOI
TL;DR: The high seroprevalence of T. gondii antibodies found in the present study suggested a widespread exposure of neotropical cats to T. Gondii in zoos in Brazil, and warrant an investigation on the mode of exposure and oocyst shedding by neotropic cats.

74 citations


Journal ArticleDOI
TL;DR: The seroprevalence study for leptospirosis in bovines and humans was realized in family holder farms along the Transamazon Highway and the serovar hardjo was the most common, followed by theserovar bratislava and grippotyphosa.
Abstract: The seroprevalence study for leptospirosis in bovines and humans was realized in family holder farms along the Transamazon Highway The prevalence of bovine leptospirosis was 97% [909 - 995%] of farms with at least one positive animal according to microscopic agglutination test for the leptospirosis diagnostic In 612% of the tested herds, the serovar hardjo was the most common, followed by the serovar bratislava (9%) and the serovar shermani (45%) The serologic prevalence of leptospirosis in humans was 328% [234 - 435%] in family groups with at least one positive individual according to microscopic agglutination test for the leptospirosis diagnostic In 9% of family groups, the serovar bratislava was the most common, while serovar hardjo and grippotyphosa accounted for 6% and 45%, respectively The impact of these results is discussed in relation to animal production and public health Suggestions have been proposed in order to improve the situation in the region

64 citations


11 Feb 2001
TL;DR: It were presented most frequent serovars of leptospires detected through Microscopic Agglutination Technique (MAT), between 1984 to 1997 in 31,325 bovines, from 1920 farms of 540 municipalities and 21 brazilian states.
Abstract: It were presented most frequent serovars of leptospires detected through Microscopic Agglutination Technique (MAT), between 1984 to 1997 in 31,325 bovines, from 1920 farms of 540 municipalities and 21 brazilian states. The MAT was conducted with a collection of 24 serovars: australis, bratislava, autumnalis, butembo, castellonis, bataviae, canicola, whitcombi, cinoptery, grippotyphosa, hebdomadis, copenhageni, icterohaemorrhagiae, javanica, panama, pomona, pyrogenes, hardjo, wolffi, shermani, tarassovi, sentot, andamana and patoc. The MAT cut off was the serum dilution at 1:100 and the tittles were obtained with a geometric serie of doble dilutions. The distribution of animals examined by estado were: SP (52,8%), MG (14,3%), RS (7,8%), PR (5,34%), RJ (3,66%), GO (3,34%), MS (2,81%), BA (2.10%), SC, (1,65%), MA (1.18%), ES (0,86%), PA (0.97%), MT (0,75%), PI (0.71%), CE (0.38%), DF (0,31%), TO (0.31%), AL (0.32%), PB (0.25%), RN (0.11%) e RO (0.07%). Thirty six and seven percent of serum samples were examined between 1984 to 1994 and 63,3% after Januray of 1995. The results showed that 84,1% of the farms; 94.18% of the municipalities and all of the states examined, it was found at least one positive animal. The proportion of reactors to at least one leptospira serovar presented the average of 37.94%. with flutuations between 25.2 to 38.3% in the States of SC, CE, PR, DF, SP and PA; from 40.7 to 58.4% PB, TO, RJ, MG, GO, RO, RN, PI, MA, AL. And higher than 61,0% in BA, ES, MS e MT. In each herd the most frequent serovar were that one wich presented the hightest title, when in the same serum sample it was found the same tittle for two or more serovars, this sample was removed from this analysis. The serovar hardjo was the most frequent in RN and DF; hardjo and wolffi in MA; AL; BA, ES, MS, SP, RJ and GO; hardjo and grippotyphosa in TO; hardjo, castellonis and patoc in SC; hardjo, wolffi and grippotyphosa in CE; hardjo

55 citations


01 Jan 2001
TL;DR: In this paper, the authors aimed to achieve a better comprehension of the epidemiological aspects of leptospirosis in the Fundacao Parque Zoologico de Sao Paulo.
Abstract: This study was aimed to achieve a better comprehension of the epidemiological aspects of leptospirosis in the Fundacao Parque Zoologico de Sao Paulo. From 1996 to 1999, blood samples were collected from 302 wild animals that were submitted to the Veterinary Division of the zoo for routine procedures. Positive Microscopic Aglutination Test were obtained from them 59 animals were positiv (19,5%) . The most frequent sorovars were copenhageni ( 15/59 = 25.4%), pomona (13/59 = 22%) and castellonis (10/59 = 16.9%). According to the orders and families of examined animals, the most frequent serovars were: Order Primate : copenhageni (13/25 = 52%), castellonis (5/25 = 20%) e grippotyphosa (2/25 = 8%). Family Felidae pomona (12/17 = 70.5%), icterohaemorrhagiae (2/17=11.7%) e grippotyphosa (1/17 = 5.8%). Family Cervidae : mini (1/1 = 100%). Family Bovidae : copenhageni (2/3 = 66.6%), pomona (1/3 = 33.3%). Order Rodentia : castellonis (2/3 = 66.6%). Family Macropodidae : sentot (1/1 = 100%). Family Girafidae : castellonis (1/1 = 100%). Free living specimens of the Muridae ( Rattus norvegicus ) and Didelphidae ( Didelphis marsupialis) families were also examined for Microscopic Aglutination Test and bacteriologic culture. Positive serologycal and bacteriologycal tests for Icterohaemorrhagiae serogrup were found in rodents, such as rats (3/7 = 42.8%) and opossum 2/5 = 40%). These strongly suggest that free-living rodents play na important role in the leptospirosis transmition inside the Zoo. Two specific areas, Extra Sector 1 and Feline Alley were considered critical due to the major proportion of seropositives animals. The possible reasons and these findings were sugestive for the profilatical procidures for the control of the Leptospirosis at the Sao Paulo Zoo were discussed.

49 citations



Journal ArticleDOI
TL;DR: Cross Agglutination Absorption Test (CAAT) was carried out with the buffalo isolate and serovar guaricura, supporting the relationship between thebuffaloes isolate and Serogroup Sejroe.

37 citations


11 Feb 2001
TL;DR: A colecao de antigenos para leptospirose utilizada incluiu 24 sorotipos, pertencentes a 20 sorogrupos de reagentes para N.caninum e Toxoplasma gondii, Brucella abortus, Lepstospira spp.
Abstract: De janeiro a maio de 1999 foram colhidas 222 amostras de sangue de bufalas em idade reprodutiva (tres a 16 anos), distribuidas em 11 propriedades destinadas a producao de carne e leite, do Vale do Ribeira, Estado de Sao Paulo, Brasil. Os soros foram submetidos a reacao de imunofluorescencia indireta (RIFI), a soroaglutinacao lenta em tubos (SAL), a soroaglutinacao microscopica (SAM) e ao ensaio imunoenzimatico (ELISA) indireto, respectivamente para pesquisa de anticorpos contra Neospora caninum e Toxoplasma gondii, Brucella abortus, Lepstospira spp. e virus BHV-1 da rinotraqueite bovina. A colecao de antigenos para leptospirose utilizada incluiu 24 sorotipos, pertencentes a 20 sorogrupos. Os resultados obtidos foram: 63,9% de reagentes para N.caninum (titulos ≥ 25), 3,2% (≥ 64) para Toxoplasma gondii, 8,6% (≥ 100) para Brucella abortus, 50,9% (≥ 100) para Leptospira spp. e 77% para virus BHV-1

14 citations


01 Jan 2001
TL;DR: The results demonstrated that in groups of calves borned from soropositive and unvaccinated cows, the colostral antibodies persisted, in mean, until the calves were 3 months old, and not even these antibody titers impeded the calves infection owing to high prevalence of BHV-1.

9 citations


Journal ArticleDOI
TL;DR: In this article, a farm in the municipality of Viradouro, in Northeast region of Sao Paulo State, was used to monitor during a period of 8 months the serum neutralizing antibody titers against BHV-1, in calves born from infected or not infected cows.
Abstract: The present study was accomplished in a farm in the municipality of Viradouro, in Northeast region of Sao Paulo State, aiming at monitoring during a period of 8 month the serum neutralizing antibody titers against BHV-1, in calves born from BHV-1 naturally infected or not infected cows. These cows were vaccinated and revaccinated against Infectious Bovine Rhinotracheitis (IBR) before parturition. The blood serum of 34 calves, separate in four groups, were analysed in this experiment. The results demonstrated that in groups of calves borned from soropositive and unvaccinated cows, the colostral antibodies persisted, in mean, until the calves were 3 months old. Calves borned from vaccinated cows, independent of these cows being infected or not, in general, presented means of antibody titers higher than the means presented by calves from unvaccinated cows. However, not even these antibody titers impeded the calves infection owing to high prevalence of BHV-1 (86.8%) in the locality of the study. The high prevalence of BHV-1 (86.8%) in the locality of the study resulted in the detection of maternal antibodies, by the serum neutralization test, in calves borned from uninfected cows, probably as a result of cows seroconversion in the interval between the screening test and parturition. The high prevalence also led to the infection of the calves from unvaccinated cows after the decline of colostral antibodies.

7 citations


Patent
13 Dec 2001
TL;DR: This paper used ABACP nucleic acid molecules and polypeptides involved in modulating seed development, stomate regulation and plant adaptation to environmental stresses such as drought, cold and a high carbon dioxide environment.
Abstract: The invention includes ABACP nucleic acid molecules and polypeptides involved in modulating seed development, stomate regulation and plant adaptation to environmental stresses such as drought, cold and a high carbon dioxide environment.

01 Jan 2001
TL;DR: The seroprevalence study for leptospirosis in bovines and humans was realized in family holder farms along the Transamazon Highway and the serovar hardjo was the most common, followed by theserovar bratislava and grippotyphosa.
Abstract: The seroprevalence study for leptospirosis in bovines and humans was realized in family holder farms along the Transamazon Highway. The prevalence of bovine leptospirosis was 97% (90.9 - 99.5%) of farms with at least one positive animal according to microscopic agglutination test for the leptospirosis diagnostic. In 61.2% of the tested herds, the serovar hardjo was the most common, followed by the serovar bratislava (9%) and the serovar shermani (4.5%). The serologic prevalence of leptospirosis in humans was 32.8% (23.4 - 43.5%) in family groups with at least one positive individual according to microscopic agglutination test for the leptospirosis diagnostic. In 9% of family groups, the serovar bratislava was the most common, while serovar hardjo and grippotyphosa accounted for 6% and 4.5%, respectively. The impact of these results is discussed in relation to animal production and public health. Suggestions have been proposed in order to improve the situation in the region.