F
Florian Hollfelder
Researcher at University of Cambridge
Publications - 208
Citations - 10356
Florian Hollfelder is an academic researcher from University of Cambridge. The author has contributed to research in topics: Biology & Chemistry. The author has an hindex of 49, co-authored 173 publications receiving 8704 citations. Previous affiliations of Florian Hollfelder include École Polytechnique Fédérale de Lausanne & Central Queensland University.
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Journal ArticleDOI
Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology
Ashleigh B. Theberge,Fabienne Courtois,Yolanda Schaerli,Martin Fischlechner,Chris Abell,Florian Hollfelder,Wilhelm T. S. Huck,Wilhelm T. S. Huck +7 more
TL;DR: Examples are presented to show how compartmentalization, monodispersity, single-molecule sensitivity, and high throughput have been exploited in experiments that would have been extremely difficult outside the microfluidics platform.
Journal ArticleDOI
Microdroplets: A sea of applications?
Ansgar Huebner,Sanjiv Sharma,Monpichar Srisa-Art,Florian Hollfelder,Joshua B. Edel,Andrew J. deMello +5 more
TL;DR: This review provides an overview of methods for generating, controlling and manipulating droplets and discusses key fields of use in which such systems may make a significant impact, with particular emphasis on novel applications in the biological and physical sciences.
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Quantitative detection of protein expression in single cells using droplet microfluidics.
Ansgar Huebner,Monpichar Srisa-Art,Daniel J. Holt,Chris Abell,Florian Hollfelder,Andrew J. deMello,Joshua B. Edel +6 more
TL;DR: It is demonstrated that single cells can be controllably compartmentalized within aqueous microdroplets and high-throughput screening is performed by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.
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Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays
Ansgar Huebner,Dan Bratton,Graeme Whyte,Min Yang,Min Yang,Andrew J. deMello,Chris Abell,Florian Hollfelder +7 more
TL;DR: The design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow is described.
Journal ArticleDOI
Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets
Yolanda Schaerli,Robert C. R. Wootton,Tom Robinson,Viktor Stein,Christopher Dunsby,Mark Andrew Aquilla Neil,Paul M. W. French,Andrew J. deMello,Chris Abell,Florian Hollfelder +9 more
TL;DR: A high throughput microfluidic device for continuous-flow polymerase chain reaction (PCR) in water-in-oil droplets of nanoliter volumes that holds promise for convenient integration with other microfluidity devices and adds a critical missing component to the laboratory-on-a-chip toolkit.