Paul M. W. French

TL;DR: This work compares different FLIM methods and finds that a chief advantage of wide-field time-gating and phase modulation methods is the speed of acquisition whereas for time-correlated single photon counting (TCSPC) based confocal scanning it is accuracy in the fluorescence decay.

TL;DR: In this article, two classes of membrane nanotubes between human monocyte-derived macrophages can be distinguished by their cytoskeletal structure and their functional properties, i.e., those > ∼ 0.7 μm in diameter, contained both F-actin and microtubules.

TL;DR: This work has applied the stretched-exponential function (StrEF) to time-domain whole-field fluorescence lifetime imaging (FLIM), yielding both excellent tissue contrast and goodness of fit using data from rat tissue, and notes that for many biological samples for which there is no a priori knowledge of multiple discrete exponential fluorescence decay profiles, the StrEF is likely to provide a truer representation of the underlying fluorescence dynamics.

TL;DR: A high throughput microfluidic device for continuous-flow polymerase chain reaction (PCR) in water-in-oil droplets of nanoliter volumes that holds promise for convenient integration with other microfluidity devices and adds a critical missing component to the laboratory-on-a-chip toolkit.

TL;DR: This work quantitatively image, by wide-field time-gated fluorescence lifetime imaging, the refractive index of the environment of GFP, and paves the way for imaging the biophysical environment of specific GFP-tagged proteins in live cells.