Florian Hollfelder

TL;DR: LFERs and KIE data for several active site mutants point to leaving group stabilization by active site K375, in cooperation with H211, and the mechanism for S-Obridge bond fission shifts, with decreasing leaving group ability, from charge compensation via Lewis acid interactions toward direct proton donation.

TL;DR: This work presents a multiparameter approach at the single-cell level that takes approximately an hour from spiking the culture to correctly classify susceptible and resistant strains and shows how this combined detection method provides insights into the activity of antimicrobials at the onset of their action which single parameter and traditional tests cannot offer.

TL;DR: The approach of producing droplets through confinement gradients and analyzing reactions within stationary drops provides an ultralow consumption platform and suggests that the platform can be automated to reach large throughput analyses of enzymes.

TL;DR: It is demonstrated that deep neural networks can accurately classify single droplet images in real-time based on the presence and number of micro-objects including single mammalian cells and multicellular spheroids.

TL;DR: In this article , a review of protease activation is presented, focusing on how recent insights into the spatial and temporal control of the proteolytic activation of growth factors and interleukins are leading to unique strategies for the discovery of new medicines.