F
Floris J. van Dalen
Researcher at Radboud University Nijmegen
Publications - 9
Citations - 186
Floris J. van Dalen is an academic researcher from Radboud University Nijmegen. The author has contributed to research in topics: Cathepsin & Medicine. The author has an hindex of 2, co-authored 4 publications receiving 99 citations.
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Journal ArticleDOI
Molecular Repolarisation of Tumour-Associated Macrophages.
Floris J. van Dalen,Marleen H. M. E. van Stevendaal,Felix L. Fennemann,Martijn Verdoes,Olga Ilina +4 more
TL;DR: This work summarises the molecular wheelwork of macrophage biology and presents an overview of molecular strategies to repolarise TAMs in immunotherapy.
Journal ArticleDOI
Dual Site-Specific Chemoenzymatic Antibody Fragment Conjugation Using CRISPR-Based Hybridoma Engineering.
Camille M. Le Gall,Johan M.S. van der Schoot,Iván Ramos-Tomillero,Melek Parlak Khalily,Floris J. van Dalen,Zacharias Wijfjes,Liyan Smeding,Duco van Dalen,Anna Cammarata,Kimberly M. Bonger,Carl G. Figdor,Ferenc A. Scheeren,Martijn Verdoes +12 more
TL;DR: In this paper, a CRISPR/Cas9-based strategy was proposed to rapidly engineer hybridoma cells to secrete Fab' fragments bearing two distinct site-specific labeling motifs, which can be separately modified by two different sortase A mutants.
Journal ArticleDOI
Enzyme‐Activatable Chemokine Conjugates for In Vivo Targeting of Tumor‐Associated Macrophages
Nicole D. Barth,Floris J. van Dalen,Utsa Karmakar,Marco Bertolini,Lorena Mendive-Tapia,Takanori Kitamura,Martijn Verdoes,Marc Vendrell +7 more
TL;DR: The first enzyme-activatable chemokine conjugates for effective targeting of defined macrophage subsets in live tumors are reported, opening new avenues in the design of targeted theranostic probes for immune cells in the tumor microenvironment.
Posted ContentDOI
Dual site-specific chemoenzymatic antibody fragment conjugation using CRISPR-based hybridoma engineering
Camille M. Le Gall,Johan M.S. van der Schoot,Iván Ramos-Tomillero,Melek Parlak Khalily,Floris J. van Dalen,Zacharias Wijfjes,Liyan Smeding,Duco van Dalen,Anna Cammarata,Kimberly M. Bonger,Carl G. Figdor,Ferenc A. Scheeren,Martijn Verdoes +12 more
TL;DR: A straightforward CRISPR/Cas9-based strategy to rapidly engineer hybridoma cells to secrete Fab’ fragments bearing two distinct site-specific labelling motifs, which can be separately modified by two different sortase A mutants are described.
Journal ArticleDOI
Application of a Highly Selective Cathepsin S Two-step Activity-Based Probe in Multicolor Bio-Orthogonal Correlative Light-Electron Microscopy
Floris J. van Dalen,Thomas Bakkum,Tyrza van Leeuwen,Mirjam Groenewold,Edgar Deu,Abraham J. Koster,Sander I. van Kasteren,Martijn Verdoes +7 more
TL;DR: In this article, the authors developed a potent and highly selective two-step activity-based probe for cathepsin S and the application in multicolor bio-orthogonal correlative light-electron microscopy.