Showing papers by "Franz Schinner published in 2011"
TL;DR: Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain C16y(T) is a representative of a novel species of the genus Sphingomonas, for which the name Spindingomonas glacialis sp.
Abstract: A non-motile, rod-shaped, yellow bacterium, designated C16yT, was isolated from alpine glacier cryoconite. Cells behaved Gram-positively, were aerobic and psychrophilic (good growth at 1–25 °C). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C16yT was related to the genus Sphingomonas and had highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica JCM 12082T (97.6 %) and Sphingomonas echinoides DSM 1805T (97.2 %). DNA–DNA hybridization demonstrated that strain C16yT could not be considered as a member of either Sphingomonas oligophenolica or Sphingomonas echinoides. Strain C16yT contained Q-10 as the predominant ubiquinone and C18 : 1 and C16 : 0 were the dominant fatty acids. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, five unidentified glycolipids, two unidentified aminophospholipids and two unidentified lipids. The major polyamines were the triamines sym-homospermidine and spermidine. The G+C content was 67.9 mol%. Combined data from phenotypic, phylogenetic and DNA–DNA relatedness studies demonstrated that strain C16yT is a representative of a novel species of the genus Sphingomonas, for which we propose the name Sphingomonas glacialis sp. nov. The type strain is C16yT (=DSM 22294T =CGMCC 1.8957T =CIP 110131T).
43 citations
TL;DR: On the basis of phenotypic characteristics and phylogenetic analysis, strainsCr9-30(T) and Cr9-12 represent a novel species in a new genus of the family Oxalobacteraceae, for which the name Glaciimonas immobilis gen. nov. is proposed.
Abstract: Strains Cr9-30T and Cr9-12 were isolated from alpine glacier cryoconite. Both strains were Gram-negative-staining, non-motile, rod-shaped and psychrophilic, showing good growth over the temperature range 1–20 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that the two strains formed a distinct branch within the family Oxalobacteraceae and were most closely related to members of the genus Collimonas. The 16S rRNA gene sequence similarity between strains Cr9-30T and Cr9-12 was 99.0 %. The two strains showed highest 16S rRNA gene sequence pairwise similarity with Collimonas pratensis LMG 23965T (96.6 and 96.1 % for strains Cr9-30T and Cr9-12, respectively), Collimonas arenae LMG 23964T (96.5 and 96.3 %, respectively) and Collimonas fungivorans LMG 21973T (96.4 and 96.2 %, respectively). The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and C18 : 1ω7c. The DNA G+C content of strain Cr9-30T was 51.0 mol%. On the basis of phenotypic characteristics and phylogenetic analysis, strains Cr9-30T and Cr9-12 represent a novel species in a new genus of the family Oxalobacteraceae, for which the name Glaciimonas immobilis gen. nov., sp. nov. is proposed. The type strain of Glaciimonas immobilis is Cr9-30T ( = DSM 23240T = LMG 25547T).
40 citations
TL;DR: On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Pseudomonas bauzanensis sp.
Abstract: A Gram-negative, aerobic, motile rod, designated BZ93T, was isolated from soil from an industrial site. The strain grew at 5–30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ93T was related to members of the genus Pseudomonas and was related most closely to Pseudomonas xiamenensis C10-2T (97.8 % 16S rRNA gene sequence similarity) and Pseudomonas pertucinogena IFO 14163T (97.4 %). The predominant cellular fatty acids of strain BZ93T were C18 : 1ω7c (54.8 %), summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH; 10.3 %), C16 : 0 (9.9 %) and C17 : 0 cyclo (7.4 %). The major quinone was ubiquinone 9. The major phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. The genomic DNA G+C content was 61.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness, a novel species, Pseudomonas bauzanensis sp. nov., is proposed. The type strain is BZ93T ( = DSM 22558T = CGMCC 1.9095T = LMG 26048T).
26 citations
TL;DR: Bacterial isolates included in Actinobacteria and Bacteroidetes were frequently isolated from pristine sites and showed low maximum growth temperature and enzyme secretion, while isolates affiliated with Enterobacteriaceae were detected only in samples from sites with strong human impact.
Abstract: The anthropogenic effect on the microbial communities in alpine glacier cryoconites was investigated by cultivation and physiological characterization of bacteria from six cryoconite samples taken at sites with different amounts of human impact. Two hundred and forty seven bacterial isolates were included in Actinobacteria (9%, particularly Arthrobacter), Bacteroidetes (14%, particularly Olleya), Firmicutes (0.8%), Alphaproteobacteria (2%), Betaproteobacteria (16%, particularly Janthinobacterium), and Gammaproteobacteria (59%, particularly Pseudomonas). Among them, isolates of Arthrobacter were detected only in samples from sites with no human impact, while isolates affiliated with Enterobacteriaceae were detected only in samples from sites with strong human impact. Bacterial isolates included in Actinobacteria and Bacteroidetes were frequently isolated from pristine sites and showed low maximum growth temperature and enzyme secretion. Bacterial isolates included in Gammaproteobacteria were more frequently isolated from sites with stronger human impact and showed high maximum growth temperature and enzyme secretion. Ecotypic differences were not evident among isolates of Janthinobacterium lividum, Pseudomonas fluorescens, and Pseudomonas veronii, which were frequently isolated from sites with different degrees of anthropogenic effect.
22 citations
TL;DR: On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain BZ78(T) is considered to represent a novel species of the genus Tistrella, for which the name Tistrell bauzanensis sp.
Abstract: A Gram-reaction-negative, strictly aerobic, motile, rod-shaped bacterium, designated strain BZ78T, was isolated from soil from an industrial site. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ78T belonged to the family Rhodospirillaceae and formed a coherent cluster with the type strain of Tistrella mobilis (98.3 % pairwise similarity). The predominant cellular fatty acids of strain BZ78T were C18 : 1ω7c (58.3 %), C19 : 0ω8c cyclo (11.5 %), C18 : 1 2-OH (10.9 %) and C14 : 0 3-OH (6.4 %). The predominant ubiquinone was Q-10. The genomic DNA G+C content of strain BZ78T was 65.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain BZ78T is considered to represent a novel species of the genus Tistrella, for which the name Tistrella bauzanensis sp. nov. is proposed. The type strain is BZ78T ( = DSM 22817T = CGMCC 1.10188T = LMG 26047T).
14 citations
TL;DR: In this paper, the potential of nitrifying and sulfuroxidizing cultures derived from rock to mobilize elements from this rock when augmented with ammonium and thiosulfate was investigated in a 35-day laboratory study.
Abstract: At a high mountain lake in catchments of mica schist and gneiss rock in the European Alps, substantial increases in solute concentrations of sulfate, magnesium, calcium, silica, manganese, and nickel were observed over the past two decades. We hypothesized that microbial interactions with rock in the catchment of the lake might play an important role. We studied the chemolithotrophic activities resulting in the production of metal mobilizing metabolites (mineral acids). The potential of nitrifying and sulfur-oxidizing cultures derived from rock to mobilize elements from this rock when augmented with ammonium and thiosulfate was investigated in a 35 day laboratory study. Bacterial species prevailing in the indigenous nitrifying and sulfur-oxidizing mixed cultures were determined by 16S rRNA gene sequence based analysis. The average mineralogical composition of the rock sample was quartz (50%), feldspar (27%), muscovite (15%), chlorite (6%), and dolomite (2%). The increase of each soluble element i...
7 citations
TL;DR: In this paper, the impact of chemolithotrophic nitrifying and sulfur-oxidizing culture enrichments on pristine mineral soil under conditions of ammonium or thiosulfate supplementation was investigated over a period of 40 days.
Abstract: Indigenous chemolithotrophic nitrifying and sulfur-oxidizing culture enrichments mobilized metals from pristine mineral soil under conditions of ammonium or thiosulfate supplementation in a laboratory experiment carried out over a period of 40 days. The average mineralogical composition of the mineral soil was quartz (62%), feldspar (20%), muscovite (6%), chlorite (2%), hornblende (2%), dolomite (4%), and calcium carbonate (4%). The leaching efficiency of the nitrifying enrichment was calcium (27%), magnesium (15%), zinc (5.4%), manganese (0.6%), and cobalt (1.4%) after 40 days of incubation. In case of sulfur-oxidizing enrichment, leaching efficiency was calcium (56%), magnesium (36%), iron (0.8%), zinc (12%), manganese (2.1%), and cobalt (12%). The impact of these organisms on pristine mineral soil could be important in understanding primary colonization and the early stages of soil formation.
5 citations
TL;DR: In this article, the potential of indigenous chemolithotrophic bacteria to mobilize metals from dolomite in the presence of added ammonium or thiosulfate was studied in a laboratory experiment over a period of 35 days.
Abstract: The potential of indigenous chemolithotrophic bacteria to mobilize metals from dolomite in the presence of added ammonium or thiosulfate was studied in a laboratory experiment over a period of 35 days. The rock sample was collected in the Dolomites (Alps, Europe). The average mineralogical composition of the sample was dolomite (89%) and calcite (11%). After 35 days, mobilization of total metal in the rock sample amounted to 3.4% (Ca), 8.5% (Mg), 1% (Al), and 0.9% (Mn) in the presence of a nitrifying culture. In the presence of a sulfur-oxidizing culture, mobilization of total magnesium in the rock sample amounted to 34%.The results contribute to our understanding of interactions between atmospheric deposition of energy substrates, bacterial activity and metal mobilization in Alpine areas.
3 citations