F
Friederike Junge
Researcher at Goethe University Frankfurt
Publications - 12
Citations - 854
Friederike Junge is an academic researcher from Goethe University Frankfurt. The author has contributed to research in topics: Membrane protein & Medicine. The author has an hindex of 9, co-authored 9 publications receiving 772 citations.
Papers
More filters
Journal ArticleDOI
Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems
Daniel Schwarz,Friederike Junge,Florian Durst,Nadine Frölich,Birgit Schneider,Sina Reckel,Solmaz Sobhanifar,Volker Dötsch,Frank Bernhard +8 more
TL;DR: This protocol describes the preparation of all essential reaction components such as the E. coli cell extract, T7 RNA polymerase, DNA templates as well as the individual stock solutions for cell-free expression.
Journal ArticleDOI
Large-scale production of functional membrane proteins
TL;DR: The review will give an overview of currently established processes for the production of functionally folded membrane proteins, a highly dynamic field where new or modified approaches are frequently emerging.
Journal ArticleDOI
Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins.
Friederike Junge,Stefan Haberstock,Christian Roos,Susanne Stefer,Davide Proverbio,Volker Dötsch,Frank Bernhard +6 more
TL;DR: An overview of the recent advances of cell-free production of membrane proteins for structural and functional analysis and the array of currently available options to modify and develop cell- free expression protocols adapted to the specific requirements of individual membrane proteins are given.
Journal ArticleDOI
Cell-free expression and stable isotope labelling strategies for membrane proteins
Solmaz Sobhanifar,Sina Reckel,Friederike Junge,Daniel Schwarz,Lei Kai,Mikhail Karbyshev,Frank Löhr,Frank Bernhard,Volker Dötsch +8 more
TL;DR: Various labelling strategies in conjunction with cell-free developments are explored, with a particular focus on α-helical transmembrane proteins which benefit most from such methods.
Book ChapterDOI
Membrane protein expression in cell-free systems.
Birgit Schneider,Friederike Junge,V.A. Shirokov,Florian Durst,Daniel Schwarz,Volker Dötsch,Frank Bernhard +6 more
TL;DR: This protocol focuses on the currently most efficient cell-free expression system for membrane proteins based on Escherichia coli extracts, and enables the production of membrane proteins in completely new modes, like the direct translation into detergent micelles, which is not possible with any other expression system.