Fulvio Gandolfi

TL;DR: Most of the examined transcripts follow the default deadenylation pattern described during oocyte maturation in other species and that a shorter poly(A) tail is correlated with low developmental competence.

TL;DR: This work shows that it is possible to convert adult fibroblasts into insulin-secreting cells, avoiding both a stable pluripotent stage and any transgenic modification.

TL;DR: To determine possible causes of reported differences between developmental competence of oocytes isolated from prepubertal calves and adult cows, three parameters were analysed, comparatively, during in vitro maturation (IVM): oocyte diameter, oocyte energy metabolism, and protein synthesis of oocyte and cumulus cells.

TL;DR: It is concluded that the maintenance of an optimal intracellular concentration of cAMP before and during IVM ensures a high developmental competence of bovine oocytes matured in medium without serum and hormones.

TL;DR: Data from the literature will be presented together with experimental evidence recently obtained in the laboratory and aspects related to the timing of isolation, the initiation of primary cultures, the use of different culture conditions and cytokines, the identification of pluripotency-related molecular markers in the pig will be examined.