G
G. M. Machado
Researcher at University of Brasília
Publications - 19
Citations - 430
G. M. Machado is an academic researcher from University of Brasília. The author has contributed to research in topics: Embryo culture & Reproductive technology. The author has an hindex of 10, co-authored 19 publications receiving 386 citations. Previous affiliations of G. M. Machado include Empresa Brasileira de Pesquisa Agropecuária.
Papers
More filters
Journal ArticleDOI
Effect of Percoll volume, duration and force of centrifugation, on in vitro production and sex ratio of bovine embryos.
G. M. Machado,J. O. Carvalho,J. O. Carvalho,E. Siqueira Filho,E. Siqueira Filho,E. S. Caixeta,E. S. Caixeta,Maurício Machaim Franco,Maurício Machaim Franco,Rodolfo Rumpf,Rodolfo Rumpf,Margot Alves Nunes Dode,Margot Alves Nunes Dode +12 more
TL;DR: Investigating the effect of Percoll volume, duration and force of centrifugation on sperm quality characteristics, embryo development, and sex ratio of in vitro-produced bovine embryos found no significant effects and the proportion of male:female embryos did not differ significantly from an expected 1:1 ratio.
Journal ArticleDOI
Quality assessment of bovine cryopreserved sperm after sexing by flow cytometry and their use in in vitro embryo production.
J. O. Carvalho,Roberto Sartori,G. M. Machado,G. M. Machado,Gerson Barreto Mourão,Margot Alves Nunes Dode,Margot Alves Nunes Dode +6 more
TL;DR: Frozen non-sexed (NS) sperm had better quality than sexed sperm, including higher motility and greater percentages of cells with an intact membrane and acrosome, and embryo development kinetics was similar.
Journal ArticleDOI
Post‐hatching development of in vitro bovine embryos from day 7 to 14 in vivo versus in vitro
G. M. Machado,G. M. Machado,A.R. Ferreira,I. Pivato,A. Fidelis,J. F. W. Sprícigo,J. F. W. Sprícigo,Fernanda Paulini,Carolina Madeira Lucci,Maurício Machaim Franco,M.A. Dode,M.A. Dode +11 more
TL;DR: It is concluded that Day‐7 IVP embryos are different from IVV embryos in regards to gene expression, although exposure to the uterine environment during the elongation period allowed theIVP embryos to overcome this difference.
Journal ArticleDOI
Methylation status in the intragenic differentially methylated region of the IGF2 locus in Bos taurus indicus oocytes with different developmental competencies.
N. S. Fagundes,Valquiria A. Michalczechen-Lacerda,Valquiria A. Michalczechen-Lacerda,E. S. Caixeta,E. S. Caixeta,G. M. Machado,G. M. Machado,F. C. Rodrigues,F. C. Rodrigues,E. O. Melo,Margot Alves Nunes Dode,Margot Alves Nunes Dode,Maurício Machaim Franco,Maurício Machaim Franco +13 more
TL;DR: The results suggest that the methylation pattern in this DMR may be a useful parameter to investigate as a molecular marker for oocyte competence in cattle and as a model for studies in other species.
Journal ArticleDOI
Vitrification of bovine oocytes at different meiotic stages using the Cryotop method: Assessment of morphological, molecular and functional patterns
J. F. W. Sprícigo,K.S. Morais,A.R. Ferreira,G. M. Machado,Alexandre Cavallieri Gomes,Rodolfo Rumpf,Maurício Machaim Franco,Margot Alves Nunes Dode,Margot Alves Nunes Dode +8 more
TL;DR: In this article, the functional, morphological and molecular patterns of bovine oocytes vitrified at different times during in vitro maturation (IVM) were investigated, and the effect of vitrification on the nuclear maturation and fertilization rates were assessed by lacmoid staining and ultrastructural electron microscopy.