Gabriela Ballesteros

TL;DR: Zika virus, chikungunya virus, and dengue virus result in similar clinical presentations, and coinfections may be relatively common.

TL;DR: A multiplex real-time reverse transcription PCR for Zika virus, chikungunya virus, and dengue virus infections to improve virus detection, streamline molecular workflow, and decrease test costs is developed.

TL;DR: A multiplex, real-time, reverse transcriptase-PCR for the detection, quantitation, and serotyping of dengue viruses in a single reaction demonstrates superior analytical and clinical performance, as well as simpler workflow compared to the hemi-nested RT-PCRs.

TL;DR: A laboratory-developed rRT-PCR proved more clinically sensitive than the FDA-approved CDC assay for DENV serotypes 1 to 4 when measured against a composite reference standard, with sensitivities of 97.4% versus 87.1%, respectively.

TL;DR: The design of an internally controlled real-time reverse transcriptase PCR (rRT-PCR) that detects all four DENV serotypes but does not distinguish between them is reported, which is believed to serve to lengthen the period during which a confirmed molecular diagnosis of DENV infection can be provided.