Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction

OVERVIEW The GRA Marketing Internship Program is offered to students who are interested in gaining valuable work experience through efforts in marketing, membership, sales, and events. Interns work directly to assist the Director of Marketing and Communications on various tasks relating to upcoming GRA events. During this internship, students will work a minimum of 10 hours a week and a maximum of 20 hours a week. Students are encouraged to earn credit for their internship, however this is a paid internship. Students interested in obtaining credit for their internship must consult their academic advisor or the intern coordinator at their academic unit.

Role of WHO.

Spectroscopy is the study of matter interacting with electromagnetic radiation (e.g., light). The electromagnetic spectrum in Figure 1 illustrates the many different types of electromagnetic radiation, including gamma rays (γ-rays), X-rays, ultraviolet (UV) radiation, visible light, infrared (IR) radiation, microwaves, and radio waves. The frequency (ν) and wavelength (λ) ranges associated with each form of radiant energy are also indicated in Figure 1.

Introduction to Spectroscopy

Recent developments in antibacterial and antifungal chitosan and its derivatives.

Tyrosinase is a multi-copper enzyme which is widely distributed in different organisms and plays an important role in the melanogenesis and enzymatic browning. Therefore, its inhibitors can be attractive in cosmetics and medicinal industries as depigmentation agents and also in food and agriculture industries as antibrowning compounds. For this purpose, many natural, semi-synthetic and synthetic inhibitors have been developed by different screening methods to date. This review has focused on the tyrosinase inhibitors discovered from all sources and biochemically characterised in the last four decades.

https://northumbria-test.eprints-hosting.org/id/document/268389

A comprehensive review on tyrosinase inhibitors.

N-substituted chitosan derivatives were synthesized through condensation with a number of selected aryl and heteroaryl aldehydes. The synthesis of the amino-derivatives has been carried out by reductive amination with sodium borohydride as reducing agent. Their structures were characterized by (FT-IR, 1HNMR, and XRD). The antimicrobial activity of Chitosan Schiff’s base (CSB) derivatives were investigated against four types of bacteria and two crop-threatening pathogenic fungi, and the results indicated that the antibacterial and antifungal activities of the investigated derivatives are very promising. Additionally, different concentrations of the triazolo-Schiff’s base derivative 3c were used for cytotoxicity screening against Human Breast Adenocarcinoma Cells (MCF-7), Human Colon Carcinoma Cells (HCT-116), and Human Hepatocellular Liver Carcinoma Cells (HepG-2), and the obtained data revealed that the examined compounds have an excellent cell growth inhibitory effects on the cell lines as compared to standard.

https://www.mdpi.com/2073-4360/7/12/1532/pdf

Synthesis and Biological Evaluation of New Imine- and Amino-Chitosan Derivatives

Chemoprotective Effect of Elettaria Cardamomum against Chemically induced Hepatocellular Carcinoma in Rats by Inhibiting NF-κB, Oxidative Stress, and Activity of Ornithine Decarboxylase

The hepatoprotective effect of basil ( Ocimum basilicum ) extract against liver fibrosis-induced by carbon tetrachloride (CCl 4 ) was studied in rats. Rats were allocated into five groups: Group I (control group); Group II [CCl 4 group; rats were injected subcutaneously with CCl 4 (1 ml/kg b.w.) twice weekly for 4 weeks (phenobarbital, 350 mg/L, was added to the drinking water throughout the experiment)]; Group III received daily oral doses of basil extract of 200 mg/kg b.w. along with CCl4 and phenobarbital for 6 weeks; Groups IV and V rats were treated with phenobarbital and CCl 4 for 6 weeks then treated daily with oral dose of 200 mg/kg b.w basil extract, or by 300 mg/kg b.w dimethyl diphenyl bicarboxylate (DDB), respectively for 6 weeks. Basil-treatment significantly reduced the liver content of hydroxyproline and significantly increased the activity of hyaluronidase (HAase). The hepatic activity of superoxide dismutase (SOD) was stimulated while the lipid peroxidation was significantly reduced by the effect of basil extract. Treatment with CCl 4 significantly increased the activities of transaminases [aspartate aminotransferase (AST), alanine aminotransferase (ALT)], and alkaline phosphatase (ALP). These activities were significantly decreased by basil extract. The higher levels of serum urea and creatinine in CCl 4 group were significantly guarded by the protection of basil. Key words : Carbon tetrachloride, liver fibrosis, antioxidant, Ocimum basilicum , dimethyl diphenyl bicarboxylate.

/pdf/hepatoprotective-effect-of-basil-ocimum-basilicum-l-on-ccl-4-17bjcbham1.pdf

Hepatoprotective effect of basil (Ocimum basilicum L.) on CCl 4 -induced liver fibrosis in rats

LPS-induced oxidative inflammation and hyperlipidemia in male rats: The protective role of Origanum majorana extract

The aim of the present study was to evaluate the diagnostic relevance of urinary fibronectin (FN), telomerase (RTA), and cytokeratin 20 (CK20) mRNA in comparison with voided urine cytology (VUC). The study included 132 patients with bladder cancer, 60 patients with benign bladder lesions, and 48 healthy individuals. All were subjected to urine cytology, estimation of fibronectin by ELISA, RTA by TRAP, and CK20 mRNA by conventional RT–PCR in urothelial cells from voided urine. The best cutoff point for FN was determined by receiver operating characteristic curve (41.7 ng/mg protein) revealed the highest sensitivity for malignant (80%) followed by the benign (70%) than the healthy individuals (4.1%) at P < 0.001. Also, RTA and VUC showed significant difference among the three investigated groups (P < 0.001). The overall sensitivity (89.3%) and specificity (98.4%) were the highest for CK20 mRNA. Combined sensitivity of VUC with FN, RTA, and CK20 mRNA together (98.4%) was higher than either the combined sensitivity of VUC with any of them or than that of the biomarker alone. Accordingly, when the diagnostic efficacy was considered, CK20 mRNA had the highest sensitivity and specificity compared to all investigated markers.

Galila A. Yacout

Papers

Synthesis and Biological Evaluation of New Imine- and Amino-Chitosan Derivatives

Chemoprotective Effect of Elettaria Cardamomum against Chemically induced Hepatocellular Carcinoma in Rats by Inhibiting NF-κB, Oxidative Stress, and Activity of Ornithine Decarboxylase

Hepatoprotective effect of basil (Ocimum basilicum L.) on CCl 4 -induced liver fibrosis in rats

LPS-induced oxidative inflammation and hyperlipidemia in male rats: The protective role of Origanum majorana extract

The clinical relevance of urine-based markers for diagnosis of bladder cancer.