Gaobin Pu

TL;DR: Transient expression experiments in agroinfiltrated Nicotiana benthamiana and A. annua leaves showed that AaWRKY1 protein transactivated the ADSpro2 promoter activity by binding to the W-box of the promoter; disruption of theW-box abolished the activation.

TL;DR: Results suggest that salicylic acid induces artemisinin biosynthesis in Artemisia annua L. annua in at least two ways: by increasing the conversion of dihydroartemisinic acid into artemis inin caused by the burst of ROS, and by up-regulating the expression of genes involved in artemisine biosynthesis.

TL;DR: Biochemical analysis demonstrated that the AabHLH1 protein was capable of binding to the E-box cis-elements, present in both ADS and CYP71AV1 promoters, and possessed transactivation activity in yeast, suggesting that Aab HLH1 can positively regulate the biosynthesis of artemisinin.

TL;DR: It is shown that foliar application of 100 mg l−1 chitosan improved artemisinin biosynthesis in A. annua and induced the expression of ADS and DBR2, which could explain the increase in level of artemisinic metabolites.

TL;DR: It is demonstrated that the inhibition pathway in the precursor competition for artemisinin biosynthesis by anti-sense technology is an effective means of increasing the art Artemisinin content of A. annua plants.