Zhigao Du

TL;DR: Transient expression experiments in agroinfiltrated Nicotiana benthamiana and A. annua leaves showed that AaWRKY1 protein transactivated the ADSpro2 promoter activity by binding to the W-box of the promoter; disruption of theW-box abolished the activation.

TL;DR: In the past decade, an Agrobacterium-mediated transformation system of A. annua is established, and a number of genes related to artemisinin biosynthesis into the plant are transferred, and another promising approach to reduce the price of art Artemisinin-based antimalarial drugs is metabolic engineering of the plant to obtain a higher content of artemisine in transgenic plants.

TL;DR: It is shown that foliar application of 100 mg l−1 chitosan improved artemisinin biosynthesis in A. annua and induced the expression of ADS and DBR2, which could explain the increase in level of artemisinic metabolites.

TL;DR: It is demonstrated that the inhibition pathway in the precursor competition for artemisinin biosynthesis by anti-sense technology is an effective means of increasing the art Artemisinin content of A. annua plants.

TL;DR: Functional and enzymatic assays showed that the recombinant enzyme was able to reversibly reduce a variety of common CADs substrates, namely geranial, cinnamyl aldehyde, sinapyl aldhyde, coniferyl aldehydes, and a sesquiterpenoid artemisinic aldechemical respectively.