Showing papers by "Gerald F. Watts published in 1987"
TL;DR: It is found that the Gimenez technique was quick, economical, and reliable in the detection of Campylobacter sp in paraffin sections of necropsy lung with L pneumophilia and other bacterial infections.
Abstract: 1964 by Gimenez.4 Greer5 used this method for identification of Legionella pneumophilia and found that it was successful in direct staining of lung smears in paraffin sections. This was attributed to processing, but it may be due to the site of the Legionella bacteria, which are intracellular. We found that the Gimenez technique was satisfactory both in smears and paraffin sections of necropsy lung with L pneumophilia and other bacterial infections, apparently staining all types of bacteria (McMullen L, personal observation). The Wartin-Starry stain, a recognised technique for the identification of spiral bacteria,6 is time consuming, expensive, and technically elaborate. The results can be unreliable due to silver precipitate, and difficulty arises in the identification of separate elements. The Gram method, although successful in direct smears of the biopsy specimen, presented problems if only a few bacteria were present. In paraffin sections the Gimenez technique was found to be superior. To detect Campylobacter in biopsy specimens of the gastrointestinal tract both bacteriology and histology should be used. The good results, however, obtained with the Gimenez method will allow studies to be performed on retrospective series. In studies of gastric antral mucosa we found that the Gimenez technique was quick, economical, and reliable in the detection of Campylobacter sp in paraffin sections.
66 citations
TL;DR: The results indicate that retinol-binding protein excretion may be increased in diabetic subjects without increased albumin excretion, and the possibility therefore exists that renal tubular damage may occur early in diabetic nephropathy without apparent glomerular dysfunction.
Abstract: A radioimmunoassay has been developed for the measurement in urine of retinol-binding protein (alpha 2-microglobulin) and used as an index of renal tubular function in adult Type 1 (insulin-dependent) diabetics and to define reference ranges in non-diabetic controls. There was a significantly greater excretion (P less than 0.001) of retinol-binding protein in the diabetic group compared to the controls in both overnight and daytime samples. There was a weak positive correlation with albumin excretion (r = 0.33; P less than 0.01) but no correlation with HbA1, duration of diabetes or arterial blood pressure. The results indicate that retinol-binding protein excretion may be increased in diabetic subjects without increased albumin excretion. The possibility therefore exists that renal tubular damage may occur early in diabetic nephropathy without apparent glomerular dysfunction.
56 citations
TL;DR: Correcting for the observed changes in serum albumin and creatinine clearance produced evidence consistent with the hypothesis that mean glomerular permeability to albumin rises progressively during pregnancy.
49 citations
TL;DR: A simplified latex bead immunoagglutination test is described employing the use of commercial antibody and is practicable, reproducible and valid in screening for ‘microalbuminuria’ in diabetic patients.
Abstract: A simplified latex bead immunoagglutination test is described employing the use of commercial antibody. It is practicable, reproducible and valid in screening for 'microalbuminuria' in diabetic patients. Although it is recommended as a side-room test its use should be supplemented where indicated by laboratory-based measurements.
9 citations