Gerald Walter

TL;DR: The polymerase chain reaction and VH family-based primers are used to clone and sequence 74 human germline VH segments from a single individual and a directory is built to include all known germline sequences.

TL;DR: Structures of the antigen binding site loops produced by nearly all the VH segments are described and it is shown that, in almost all cases, the residue patterns in the Vh segments imply that the first hyper variable regions have one of three different canonical structures and that the second hypervariable regions haveOne of five different canonical Structures.

TL;DR: This application would not be restricted to antigen-antibody systems, protein microarrays should provide a general resource for high-throughput screens of gene expression and receptor-ligand interactions.

TL;DR: A technique to establish catalogues of protein products of arrayed cDNA clones identified by DNA hybridisation or sequencing is developed and two example genes, GAPDH and HSP90alpha, were identified on high-density filters using DNA probes and antibodies against their proteins.

TL;DR: A map of the immunoglobulin VH locus which accounts for almost all VH segments known to rearrange in B-lymphocytes is completed, demonstrating the importance of combinatorial diversity produced by VDJ joining and the association of heavy and light chains in producing the human antibody repertoire.