Gerd Sutter

TL;DR: Deletion of the viral IL1betaR gene may be considered a relevant approach to amplify the virus-specific CD8+ memory T-cell response and duration of protective immunity obtained after MVA vaccination.

TL;DR: This chapter provides state-of-the-art protocols for generation, plaque isolation, molecular characterization, as well as amplification and purification of MVA vector viruses to obtain recombinant viruses for further evaluation.

TL;DR: This work reports on easily obtaining recombinant MVA using stringent growth selection on rabbit kidney RK-13 cells and describes the construction and use of new MVA vector plasmids that carry an expression cassette of the vaccinia virus host range gene, K1L, as a transient selectable marker.

TL;DR: A system which allows the reconstitution of measles virus (MV) from cloned cDNA is described and is especially suitable for studying the functions of N, P and L.

TL;DR: Strong cellular immune responses and reduction of challenge virus burden were demonstrated in animals immunized i.m. and i.r.o. only following homologous intravenous simian-human immunodeficiency virus (SHIV) challenge, and the breadth and magnitude of the induced immune responses correlated with protective efficacy.