Giovanni Candiano

TL;DR: A modified Neuhoff's colloidal Coomassie Blue G‐250 stain is reported, dubbed “blue silver” on account of its considerably higher sensitivity, approaching the one of conventional silver staining, and its full compatibility with mass spectrometry of eluted polypeptide chains is confirmed.

TL;DR: The data support AR and SOD2 as renal antigens of human MN and suggest that oxidative stress may drive glomerular SOD1 expression, while preliminary in vitro experiments showed an increase of S OD2 expression on podocyte plasma membrane after treatment with hydrogen peroxide.

TL;DR: The pretransplantation and posttransplantation outcomes in the group of patients with mutations of the podocin gene are similar to classical idiopathic FSGS, including the possibility of recurrence of proteinuria that is mild and responsive to plasmapheresis.

TL;DR: In this paper, Wu et al. used two-dimensional electrophoresis in soft gel, Western blot, and matrix-assisted laser desorption/ionization time of flight mass spectrometry to analyze 23 patients with different idiopathic nephrotic syndromes (10 steroid-sensitive minimal-change nephropathy, seven steroid-resistant FSGS, and six membranous glomerulonephritis) to reveal unexpected structural and functional aspects of proteinuria that may play a key role in pathogenesis.

TL;DR: The data suggest that IgG(4) is the prevalent isotype for antibodies against cytoplasmic antigens of podocytes that were higher than in other proteinuric glomerulonephritides and in normal controls and were correlated with anti-PLA2r.