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Glenn Thomas Horn
Researcher at Cetus Corporation
Publications - 33
Citations - 36563
Glenn Thomas Horn is an academic researcher from Cetus Corporation. The author has contributed to research in topics: Nucleic acid & Nucleic acid sequence. The author has an hindex of 16, co-authored 33 publications receiving 36001 citations. Previous affiliations of Glenn Thomas Horn include Hoffmann-La Roche.
Papers
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Journal ArticleDOI
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Randall Keichi Saiki,Stephen J. Scharf,Fred A. Faloona,Kary B. Mullis,Glenn Thomas Horn,Henry A. Erlich,Norman Arnheim +6 more
TL;DR: Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia, using primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase of target DNA copies.
Journal ArticleDOI
Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
Kary B. Mullis,Fred A. Faloona,Stephen J. Scharf,Randall Keichi Saiki,Glenn Thomas Horn,Henry A. Erlich +5 more
TL;DR: An alternative method for the synthesis of specific DNA sequences is explored that involves the reciprocal interaction of two oligonucleotides and the DNA polymerase extension products whose synthesis they prime, when they are hybridized to different strands of a DNA template in a relative orientation such that their extension products overlap.
Journal ArticleDOI
Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.
TL;DR: The polymerase chain reaction (PCR) procedure is used to enzymatically amplify a specific segment of the β-globin or HLA-DQα gene in human genomic DNA before hybridization with ASOs, enabling the analysis of allelic variation with as little as 1 ng of genomic DNA and the use of a simple ‘dot blot’ for probe hybridization.
Patent
Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
TL;DR: In this paper, a process for amplifying any target nucleic acid sequence contained in a mixture of nucleic acids or mixture thereof is described, which involves treating separate complementary strands of the nucleic acyclic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic amino acid sequence.