In the current scenario, it is an urgent requirement to satisfy the nutritional demands of the rapidly growing global population. Using conventional farming, nearly one-third of the crops get damaged mainly due to pest infestation, microbial attacks, natural disasters, poor soil quality, and lesser nutrient availability. More innovative technologies are immediately required to overcome these issues. In this regard, nanotechnology has contributed to the agrotechnological revolution that has imminent potential to reform the resilient agricultural system, while promising food security. Therefore, nanoparticles are becoming a new-age material to transform modern agricultural practices. The variety of nanoparticles-based formulations, including, nano-sized pesticides, herbicides, fungicides, fertilizers, and sensors, have been widely investigated for plant health management and soil improvement. In-depth understanding of plant and nanomaterial interactions opens new avenues towards improving crop practices through increased properties like disease resistance, crop yield, and nutrient utilization. In this review, we highlight the critical points to address the current nanotechnology-based agricultural research that could benefit productivity and food security in future.

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Nanoparticle-Based Sustainable Agriculture and Food Science: Recent Advances and Future Outlook

Copper bioavailability, uptake, toxicity and tolerance in plants: A comprehensive review.

The progressive increase in nanoparticles (NPs) applications and their potential release into the environment because the majority of them end up in the soil without proper care have drawn considerable attention to the public health, which has become an increasingly important area of research. It is required to understand ecological threats of NPs before applications. Once NPs are released into the environment, they are subjected to translocation and go through several modifications, such as bio/geo-transformation which plays a significant role in determination of ultimate fate in the environment. The interaction between plants and NPs is an important aspect of the risk assessment. The plants growing in a contaminated medium may significantly pose a threat to human health via the food chain. Metal oxide NPs ZnO and CuO, the most important NPs, are highly toxic to a wide range of organisms. Exposure and effects of CuO and ZnO NPs on soil biota and human health are critically discussed in this study. The potential benefits and unintentional dangers of NPs to the environment and human health are essential to evaluate and expected to produce less toxic and more degradable NPs to minimize the environmental risk in the future.

ZnO and CuO nanoparticles: a threat to soil organisms, plants, and human health.

This review presents a perspective on the research trends and solutions from recent years in the domain of antimicrobial packaging materials. The antibacterial, antifungal, and antioxidant activities can be induced by the main polymer used for packaging or by addition of various components from natural agents (bacteriocins, essential oils, natural extracts, etc.) to synthetic agents, both organic and inorganic (Ag, ZnO, TiO2 nanoparticles, synthetic antibiotics etc.). The general trend for the packaging evolution is from the inert and polluting plastic waste to the antimicrobial active, biodegradable or edible, biopolymer film packaging. Like in many domains this transition is an evolution rather than a revolution, and changes are coming in small steps. Changing the public perception and industry focus on the antimicrobial packaging solutions will enhance the shelf life and provide healthier food, thus diminishing the waste of agricultural resources, but will also reduce the plastic pollution generated by humankind as most new polymers used for packaging are from renewable sources and are biodegradable. Polysaccharides (like chitosan, cellulose and derivatives, starch etc.), lipids and proteins (from vegetal or animal origin), and some other specific biopolymers (like polylactic acid or polyvinyl alcohol) have been used as single component or in blends to obtain antimicrobial packaging materials. Where the package’s antimicrobial and antioxidant activities need a larger spectrum or a boost, certain active substances are embedded, encapsulated, coated, grafted into or onto the polymeric film. This review tries to cover the latest updates on the antimicrobial packaging, edible or not, using as support traditional and new polymers, with emphasis on natural compounds.

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Biodegradable Antimicrobial Food Packaging: Trends and Perspectives

Ischemic stroke is the leading cause of human disability and mortality in the world. The main problem in stroke therapy is the search of efficient neuroprotector capable to rescue neurons in the potentially salvageable transition zone (penumbra), which is expanding after brain damage. The data on molecular mechanisms of penumbra formation and expression of diverse signaling proteins in the penumbra during first 24 h after ischemic stroke are discussed. Two basic features of cell death regulation in the ischemic penumbra were observed: (1) both apoptotic and anti-apoptotic proteins are simultaneously over-expressed in the penumbra, so that the fate of individual cells is determined by the balance between these opposite tendencies. (2) Similtaneous and concerted up-regulation in the ischemic penumbra of proteins that execute apoptosis (caspases 3, 6, 7; Bcl-10, SMAC/DIABLO, AIF, PSR), signaling proteins that regulate different apoptosis pathways (p38, JNK, DYRK1A, neurotrophin receptor p75); transcription factors that control expression of various apoptosis regulation proteins (E2F1, p53, c-Myc, GADD153); and proteins, which are normally involved in diverse cellular functions, but stimulate apoptosis in specific situations (NMDAR2a, Par4, GAD65/67, caspase 11). Hence, diverse apoptosis initiation and regulation pathways are induced simultaneously in penumbra from very different initial positions. Similarly, various anti-apoptotic proteins (Bcl-x, p21/WAF-1, MDM2, p63, PKBα, ERK1, RAF1, ERK5, MAKAPK2, protein phosphatases 1α and MKP-1, estrogen and EGF receptors, calmodulin, CaMKII, CaMKIV) are upregulated. These data provide an integral view of neurodegeneration and neuroprotection in penumbra. Some discussed proteins may serve as potential targets for anti-stroke therapy.

Apoptosis regulation in the penumbra after ischemic stroke: expression of pro- and antiapoptotic proteins

Toxicity of copper oxide nanoparticles on spring barley (Hordeum sativum distichum).

After ischemic stroke, cell damage propagates from infarct core to surrounding tissues (penumbra). To reveal proteins involved in neurodegeneration and neuroprotection in penumbra, we studied protein expression changes in 2-mm ring around the core of photothrombotic infarct induced in the rat brain cortex by local laser irradiation after administration of Bengal Rose. The ultrastructural study showed edema and degeneration of neurons, glia, and capillaries. Morphological changes gradually decreased across the penumbra. Using the antibody microarrays, we studied changes in expression of >200 neuronal proteins in penumbra 4 or 24 h after focal photothrombotic infarct. Diverse cellular subsystems were involved in the penumbra tissue response: signal transduction pathways such as protein kinase Bα/GSK-3, protein kinase C and its β1 and β2 isoforms, Wnt/β-catenin (axin1, GSK-3, FRAT1), Notch/NUMB, DYRK1A, TDP43; mitochondria quality control (Pink1, parkin, HtrA2); ubiquitin-mediated proteolysis (ubiquilin-1, UCHL1); axon outgrowth and guidance (NAV-3, CRMP2, PKCβ2); vesicular trafficking (syntaxin-8, TMP21, Munc-18-3, synip, ALS2, VILIP1, syntaxin, synaptophysin, synaptotagmin); biosynthesis of neuromediators (tryptophan hydroxylase, monoamine oxidase B, glutamate decarboxylase, tyrosine hydroxylase, DOPA decarboxylase, dopamine transporter); intercellular interactions (N-cadherin, PMP22); cytoskeleton (neurofilament 68, neurofilament-M, doublecortin); and other proteins (LRP1, prion protein, β-amyloid). These proteins are involved in neurodegeneration or neuroprotection. Such changes were most expressed 4 h after photothrombotic impact. Immunohistochemical and Western blot studies of expression of monoamine oxidase B, UCHL1, DYRK1A, and Munc-18-3 confirmed the proteomic data. These data provide the integral view on the penumbra response to photothrombotic infarct. Some of these proteins can be potential targets for ischemic stroke therapy.

Protein Profile and Morphological Alterations in Penumbra after Focal Photothrombotic Infarction in the Rat Cerebral Cortex

Effects of Cu toxicity from contaminated soil were analysed in spring barley (Hordeum sativum distichum), a widely cultivated species in South Russia. In this study, H. sativum was planted outdoors in one of the most fertile soils-Haplic Chernozem spiked with high concentration of Cu and examined between the boot and head emergence phase of growth. Copper toxicity was observed to cause slow ontogenetic development of plants, changing their morphometric parameters (shape, size, colour). To the best of our knowledge, the ultrastructural changes in roots, stems and leaves of H. sativum induced by excess Cu were fully characterized for the first time using transmission electron microscopy. The plant roots were the most effected, showing degradation of the epidermis, reduced number of parenchyma cells, as well as a significant decrease in the diameter of the stele and a disruption and modification to its cell structure. The comparative analysis of the ultrastructure of control plants and plants exposed to the toxic effects of Cu has made it possible to reveal significant disruption of the integrity of the cell wall and cytoplasmic membranes in the root with deposition of electron-dense material. The changes in the ultrastructure of the main cytoplasmic organelles-endoplasmic reticulum, mitochondria, chloroplasts and peroxisomes-in the stem and leaves were found. The cellular Cu deposition, anatomical and ultrastructural modifications could mainly account for the primary impact points of metal toxicity. Therefore, this work extends the available knowledge of the mechanisms of the Cu effect tolerance of barley.

Anatomical and ultrastructural responses of Hordeum sativum to the soil spiked by copper

Autophagy in flower petal epidermal cells is a little studied process. However, it is of interest regarding the physiology of chlorophylless plant tissues. We show that the utilization of organelles in the Petunia hybrida L. flower petal epidermis occurs via the autophagic-like organelle targeting into vacuole during the particular process of vacuole fragmentation and growth of secondary vacuoles (ATVF). It differs from macroautophagy (formation of autophagosome) and microautophagy. Peroxisomes were shown to be subjected to both macroautophagy (as single microbodies and their assemblies) and to ATVF when they are in complexes with mitochondria and chromoplasts. In addition, we have demonstrated that ATVF in flower petals is accompanied with the sequestration of the cytoplasmic sectors located between the neighboring cell wall ingrowths, the ultrastructure of which has been reported in the present work for the first time. It has been shown there are more numerous mitochondria, a more pronounced ATVF, and a thicker cytoplasmic layer in the blue tissues, possibly reflecting more intensive metabolic processes in them compared to white tissues. We assume that these differences may be of interest for the further studies and may be discussed including in terms of anthocyanin-dependent photochemical effects.

Grigory Fedorenko

Papers

Toxicity of copper oxide nanoparticles on spring barley (Hordeum sativum distichum).

Protein Profile and Morphological Alterations in Penumbra after Focal Photothrombotic Infarction in the Rat Cerebral Cortex

Anatomical and ultrastructural responses of Hordeum sativum to the soil spiked by copper

Targeting of organelles into vacuoles and ultrastructure of flower petal epidermis of Petunia hybrida