G
Guilherme N. M. Ferreira
Researcher at Instituto Superior Técnico
Publications - 13
Citations - 982
Guilherme N. M. Ferreira is an academic researcher from Instituto Superior Técnico. The author has contributed to research in topics: Plasmid & Plasmid preparation. The author has an hindex of 10, co-authored 13 publications receiving 962 citations.
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Journal ArticleDOI
Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks
Duarte M. F. Prazeres,Guilherme N. M. Ferreira,Gabriel A. Monteiro,Charles L. Cooney,Joaquim M. S. Cabral +4 more
TL;DR: There are several problems and bottlenecks associated with the design and operation of large-scale processes for the production of pharmaceutical-grade plasmid DNA for gene therapy.
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Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications.
TL;DR: A review of downstream operations for large-scale purification of plasmid DNA, describing their principles and the strategy used to attain a final product that meets specifications is presented in this article.
Journal ArticleDOI
Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography.
Maria Margarida Diogo,João A. Queiroz,Gabriel A. Monteiro,Gabriel A. Monteiro,S. A. M. Martins,S. A. M. Martins,Guilherme N. M. Ferreira,Duarte M. F. Prazeres +7 more
TL;DR: A new method is described for the purification of a cystic fibrosis plasmid vector of clinical grade, which includes an ammonium sulfate precipitation followed by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatized with 1,4‐butanediol‐diglycidylether.
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Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications.
TL;DR: It is indicated that downstream processing times and costs can be considerably reduced without affecting plasmid purity, and several studies undertaken during the development of process flow‐sheets for the downstream processing of supercoiled plasmids are described.
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Anion exchange purification of plasmid DNA using expanded bed adsorption.
TL;DR: Expanded bed anion exchange chromatography was evaluated as an alternative to fixed bed chromatography for the purification of pharmaceutical-grade plasmid DNA and found both recovery yield and purity abruptly decreased when larger amounts were processed.