Showing papers by "Guleray Agar published in 2010"
TL;DR: All hexane extracts investigated in the present study can be considered genotoxically safe because they do not have mutagenic activity at the tested concentrations, and a great many of them have antimutagenic activities against 9‐Aminoacridine known as a model intercalator agent.
Abstract: Medical plants and their various extracts have been occasionally used in the treatment of many diseases. Astragalus is one of those medical plants and it has several biological activities. In the present study, the hexane extracts of six Astragalus species, which are grown in the eastern Anatolia region of Turkey, were isolated, and their mutagenic and antimutagenic properties were investigated by using Salmonella typhimurium TA1535, TA1537 and Escherichia coli WP2uvrA tester strains at 0.05, 0.5 and 5 microg/plate concentrations. Known mutagens sodium azide (NaN(3)), 9-Aminoacridine (9-AA) and N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) were used to determine antimutagenic properties of hexane extracts. The results showed that all hexane extracts, investigated in the present study, can be considered genotoxically safe because they do not have mutagenic activity at the tested concentrations. But, a great many of them have antimutagenic activity against 9-Aminoacridine known as a model intercalator agent. The inhibition rates obtained from the antimutagenicity assays ranged from 27.51% (A. macrocephalus--0.05 microg/plate) to 54.39% (A. galegiformis--5 microg/plate). These activities are valuable toward an extension of the employ of these drugs as new phytotherapeutic or preservative ingredients.
52 citations
01 Jan 2010
29 citations
TL;DR: The results show that these natural compounds have an ability to reduce or prevent the effects of these mutagenic substances.
Abstract: We aimed to determine the mutagenic, antimutagenic and antigenotoxic effects ofRhizoplaca chrysoleuca and Rhizoplaca melanophthalma's methanol extracts on the known mutagens against two different organisms using Mitotic Index (MI) and Ames-Salmonella assay systems. For the MI assay the genotoxic dose of NaN3was defined on Zea mays seeds and different dose of the lichen extract used as anti-mutagen (5, 10, 20, 40 μg/plate). Observed data showed that methanol extracts prevent the cytotoxic effect of NaN3 partially. In addition, the antimutagenic activities of the methanol extracts were investigated against 9-AA in TA1537 and NaN3 in TA1535 strains of Salmonella typhimurium. Extracts show antimutagenic effect against 9-AA-induced mutation in TA1537 strain at all tested concentrations. The inhibition rates ranged from 70.73 to 85.71% (R. chrysoleuca, 0.5 μg/plate - 5 μg/plate). The results show that these natural compounds have an ability to reduce or prevent the effects of these mutagenic substances.
Key words: Ames, Salmonella, antimutagenicity, genotoxicity, mitotic index,Rhizoplaca chrysoleuca, Rhizoplaca melanophthalma.
18 citations
TL;DR: The genetic variation and relationships among 11 turnip genotypes sampled from diverse environments of the Erzurum region located in Eastern Anatolia in Turkey are examined, concluding that RAPD analysis would be useful for genotyping turnIP genotypes.
Abstract: Turnip (Brassica rapa var. rapa) is one of the main vegetables consumed by people living in Eastern Anatolia in Turkey. In this region, farmers obtain their own seeds for production, which results in considerable morphological variability. We examined the genetic variation and relationships among 11 turnip genotypes sampled from diverse environments of the Erzurum region located in Eastern Anatolia in Turkey. Thirty-two Operon RAPD primers were screened; among them, 20 gave reproducible and clear DNA fragments after amplification. The average polymorphism ratio was 90.4%. The genetic distance between turnip genotypes were found to range from 0.302 to 0.733, indicating high genetic variability. Eleven genotypes were divided into three main clusters in a dendrogram; ETS2 and ETS8 genotypes were the most distant. We conclude that RAPD analysis would be useful for genotyping turnip genotypes.
13 citations
TL;DR: During the germination of the legume seeds, a noticeable increase was determined only in peroxidase activity in two types of the old seeds, while catalase activity decreased, however, the other biochemical parameters studied did not significantly change between the germinating old seeds and their freshly harvested controls.
Abstract: This study was carried out to evaluate the effect of long-term natural aging on germinability and several biochemical characteristics regarding antioxidative response of both dry and germinating two different clover (Trifolium repens and Trifolium pratense) seeds stored for 40 years. The percent germination of the seeds was monitored for 7 days. The activities of catalase, peroxidase, superoxide dismutase, lipid peroxidation, H2O2 levels, and phenolic matter content were tested on 0, 1st, 3rd, and 7th days of germination. On the 7th day of germination, the germination ratios of the old T. repens and T. pratense seeds were 32 and 17%, while freshly harvested seeds showed 99 and 96% germination on the 4th day, respectively. The long-term aging caused an important increase in lipid peroxidation levels of the old dry seeds. Total phenolic content was high in the old dry seeds of T. repens compared with those of T. pratense. Remarkably, the long-term aging caused an important decrease in H2O2 content ...
11 citations
TL;DR: Turkey is one of the most important genetic resources of the date plum, Diospyros lotus, especially in the northeastern part of the country, and Authenticating the identity of germplasm resources of D. lotus would be of great value for breeding.
Abstract: Turkey is one of the most important genetic resources of the date plum, Diospyros lotus, especially in the northeastern part of the country. Authenticating the identity of germplasm resources of D. lotus would be of great value for breeding. We examined the genetic variability of 11 D. lotus genotypes sampled from Coruh Valley in Turkey. One hundred and twenty-eight DNA markers were generated by 12 random primers. The highest polymorphism ratio was observed with the primer OPA-01 (71%) while the lowest was with OPY-01 (36%). The band size was between 350 and 2500 bp for these primers. The percentage of polymorphic bands was 58%, which demonstrated the efficiency of these primers. The similarity between genotypes ranged from 0.48 to 0.76. The RAPD markers permitted us to distinguish all the genotypes.
10 citations
Journal Article•
TL;DR: The results of the RAPD analysis suggest that the highest percentage of polymorphic loci was recorded in pine population, which was followed by the campus and colonizing populations, while pine and campus populations are the farthest.
Abstract: The present study employed FAMEs and RAPD to investigate the patterns of biochemical and genetic variation among and within three different populations of Vicia canescens L. Fourteen primers were used for the RAPD and polymorphic bands were obtained with eight primers. Biochemical and genetic distances were calculated and dendrograms were obtained for all studied populations using both RAPD and FAMEs methods. The results of our RAPD analysis suggest that the highest percentage of polymorphic loci (63.7%) was recorded in pine population, which was followed by the campus (60.8%) and colonizing (43.1%) populations. A UPGMA analysis was carried out using Jaccard's index of similarity for 45 individuals in the three populations, which produced a dendrogram. The index revealed that the campus and colonizing populations are the closest ones, while pine and campus populations are the farthest.
3 citations
TL;DR: Genetic variation and structure of six natural populations of Lepidium draba L. from Eastern Anatolia were assessed using random amplified polymorphic DNA (RAPD) markers, indicating that most genetic diversity occurs within populations.
Abstract: Genetic variation and structure of six natural populations of Lepidium draba L. from Eastern Anatolia were assessed using random amplified polymorphic DNA (RAPD) markers. For RAPD analysis, 12 primers generated 218 reproducible bands across the six populations analyzed, of which 73 bands (33.3%) were polymorphic. The mean Nei’s gene diversity value for all six populations was 0.1771. Shannon’s information index varied with population (0.2278–0.3082), averaging 0.2608. Analysis of molecular variance (AMOVA) showed that genetic diversity was greater within populations (58.66%) than among populations (30.68%). In addition, the variation between groups was 10.33%. The genetic differentiation among populations (G
ST) was 0.3210, indicating that most genetic diversity occurs within populations. Gene flow (Nm) was low, at only 0.5288.
2 citations
TL;DR: It appears that both RAPD and FA analyses are useful for differentiation of Astragalus species, and four genetically distinct groups were found among the species.
Abstract: Fatty acid (FA) and RAPD profiles were used to examine phenotypic and genotypic relationships, respectively among Astragalus atrocarpus , A. campylosema subsp. campylosema , A. fumosus , A. galegiformes , A. lineatus var. jildisianus and A. trichostigma . All the taxa were separated based on the presence and composition of 33 different FAs. A set of nine FAs (14 : 0, 16 : 0, 18 : 0, 18 N alcohol, 17 : 1 iso G, 18: 1 : ω8c, C18 : 1 : ω9t alcohol, 18 : 2 : ω6c, 19 : 1: ω6c) were found in all Astragalus taxa. Six of the ten decamer primers examined were selected to explore the genetic variation among the taxa tested. The RAPD results suggested that A. fumosus , A. lineatus and A. trichostigma are closely related and completely different from the rest of the taxa. Four genetically distinct groups were found among the species. High genetic variations were present among species growing wildly in eastern Anatolia region of Turkey, which may imply differences in their origin. It appears that both RAPD and FA analyses are useful for differentiation of Astragalus species. Keywords: Astragalus spp.; Genotypic; Phenotypic; Diversity; FAMEs; RAPD; Turkey DOI: 10.3329/bjb.v39i1.5518 Bangladesh J. Bot. 39(1): 1-7, 2010 (June)
1 citations