Post-stroke depression (PSD) is a mood disorder characterized by depression and anhedonia caused by stroke. Metabolomics identified metabolites associated with PSD, but previous studies are based on gas chromatography (GC)/mass spectrometry (MS). This study aimed to perform a liquid chromatography (LC)-MS-based metabolomics study of the plasma metabolite profiles between patients with PSD and controls. This was a prospective study of patients with stroke enrolled between July and December 2017 at the Second Affiliated Hospital of Nanchang University. Patients were grouped as Hamilton Depression Rating Scale > 7 (PSD) or   0.05). There were six differential metabolites between the PSD and stroke groups, of which three metabolites were increased and three were decreased. Compared with the control group, p-chlorophenylalanine (Log2FC = 1.37, P = 0.03), phenylacetyl glutamine (Log2FC = 0.21, P = 0.048), and DHA (Log2FC = 0.77, P = 0.01) levels were higher in the PSD group, while betaine (trimethylglycine) (Log2FC = − 0.79, P = 0.04), palmitic acid (Log2FC = − 0.51, P = 0.001), and MHPG-SO4 (Log2FC = − 2.37, P = 0.045) were decreased. Plasma metabolomics showed that amino acid metabolism (phenylacetyl glutamine, p-chlorophenylalanine, trimethylglycine), lipid metabolism (DHA, palmitic acid, trimethylglycine), and oxidative stress (DHA, palmitic acid, trimethylglycine) were associated with PSD. These results could help to reveal the pathophysiological mechanism of PSD and eventually identify treatment targets.

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Amino acid metabolism, lipid metabolism, and oxidative stress are associated with post-stroke depression: a metabonomics study.

Objective To analysis the characteristics of proton magnetic resonance spectroscopy(1H-MRS) on hippocampus in patients with post-stroke depression. Methods A total of 32 patients with post-stroke depression( research group),30 patients with stroke( case-control group) and 30 normal controls( control group) were assessed in this study. A multi-voxel1H-MRS was given to all the subjects on hippocampus within 24 hours they got in hospital. The NAA,Cho and Cr were measured and the ratios of NAA / Cr and Cho / Cr were determined. Results In left and right hippocampus, the NAA / Cr ratio in research group demonstrated lower than that in other two groups. In left hippocampus,the Cho / Cr ratio in research group showed higher than that in other two groups( P 0. 05); in right hippocampus,the Cho / Cr ratio was higher than that in other two groups( P 0. 05). In left and right hippocampus,the NAA / Cr ratio in the severe depression group was lower than that in the mild depression group( P 0. 05),there was no significant difference in the Cho / Cr ratio among the different degree of depression( P 0. 05). In left and right hippocampus,the NAA / Cr ratio in research group showed a significant negative correlation with the HAMD scores( P 0. 05); the Cho / Cr ratio not showed a significant correlation with The HAMD scores( P 0. 05). In the three groups,there were no significant differences in NAA / Cr and Cho / Cr between bilateral hippocampus( P 0. 05). Conlusions Abnormal metabolism of hippocampal neurons maybe the neurobiological basis of post-stroke depression. There is no significantly lateralized asymmetry for metabolite levels such as NAA,Cho among post-stroke depression,stroke patients and normal subjects.

Guo Zhi-yon

Papers

Analysis of proton magnetic resonance spectroscopy imaging on hippocampus in patients with post-stroke depression