Showing papers by "Hakon Leffler published in 1995"
TL;DR: The complete structure of β-eliminated chains of GlyCAM-1 is defined using metabolic radiolabeling, plant lectin binding, and glycosidase digestions in conjunction with high pH anion-exchange chromatography.
291 citations
TL;DR: Structural findings suggest a potential intracellular and/or extracellular role(s) for the galectin-associated activities of CLC protein in eosinophil and basophil function in allergic diseases and inflammation.
138 citations
TL;DR: Galectin-5 is a weak agglutinin of rat erythrocytes, despite its monomeric structure, and the cDNA encoding it has been isolated from a rat reticulocyte cDNA library.
110 citations
TL;DR: This chapter focuses on the identification of bacterial receptor proteins and quantifying strength of interactions that they mediate, and the force by which bacteria bind to the receptors is quantified by determining the centrifugal force required to break the interaction.
Abstract: Publisher Summary This chapter focuses on the identification of bacterial receptor proteins and quantifying strength of interactions that they mediate One of the most difficult problems encountered in studying the molecular basis of bacterial adhesion is determining which component of a complex mixture, such as a biological fluid or a cell membrane, can act as a receptor Two assays have been developed that can be used to gain important information concerning bacterial receptors An analogous assay has been developed in which proteins are separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE), blotted onto nitrocellulose, and overlaid with radiolabeled bacteria; components to which the bacteria bind are then identified by autoradiography This technique is used to study interactions between salivary glycoproteins and various species of bacteria that colonize the oral cavity Once an interaction is identified, its biological significance depends on whether structurally related endogenous receptors are present at the site of infection in vivo and whether the strength of adhesion is greater than that of distractive forces In the second method, the force by which bacteria bind to the receptors is quantified by determining the centrifugal force required to break the interaction Modifications to both protocols can be used to gain even more information about the interactions detected
5 citations
TL;DR: This chapter provides an example of studies at each level of studies on microbial adhesion and discusses the implications for what might occur in vivo.
Abstract: Publisher Summary The ultimate goal of studies on microbial adhesion is to understand what molecular interactions between the host and microbe occur in vivo and the impact of these interactions on disease processes With this goal in mind, the problem can be approached at four levels At the biochemical level, the host receptors at the relevant colonization site are identified; at the cell biology level, consequences of bacterial binding to host epithelial cells are studied in cell culture; at the physiological level, the consequences of bacterial binding are studied in experimental animals or humans; and at the population level, the consequences of receptor binding for colonization are studied by epidemiological methods This chapter provides an example of studies at each level and discusses the implications for what might occur in vivo
3 citations