H
Hameer Ruparel
Researcher at Columbia University
Publications - 8
Citations - 960
Hameer Ruparel is an academic researcher from Columbia University. The author has contributed to research in topics: Single molecule real time sequencing & DNA sequencing. The author has an hindex of 7, co-authored 8 publications receiving 938 citations.
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Journal ArticleDOI
Click chemistry to construct fluorescent oligonucleotides for DNA sequencing.
TL;DR: The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.
Journal ArticleDOI
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Tae Seok Seo,Xiaopeng Bai,Dae H. Kim,Qinglin Meng,Shundi Shi,Hameer Ruparel,Zengmin Li,Nicholas J. Turro,Jingyue Ju +8 more
TL;DR: It is demonstrated that photocleavable fluorescent nucleotide analogues can be incorporated accurately into a growing DNA strand during a polymerase reaction in solution and on a chip and all four fluorophores can be detected and efficiently cleaved using near-UV irradiation, thereby allowing continuous identification of the DNA template sequence.
Journal ArticleDOI
Design and synthesis of a 3′-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis
TL;DR: After the nucleotide was successfully incorporated into a growing DNA strand and the fluorophore was photocleaved, the allyl group was removed by using a Pd-catalyzed reaction to reinitiate the polymerase reaction, thereby establishing the feasibility of using such nucleotide analogues as reversible terminators for SBS.
Journal ArticleDOI
A photocleavable fluorescent nucleotide for DNA sequencing and analysis
TL;DR: It is demonstrated that the nucleotide analogue can be faithfully incorporated by a DNA polymerase Thermo Sequenase into the growing DNA strand in a DNA-sequencing reaction and that its incorporation does not hinder the addition of the subsequent nucleotide.
Journal ArticleDOI
Mass-spectrometry DNA sequencing.
TL;DR: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been explored widely for DNA sequencing and more recently, assays have been developed to indirectly sequence DNA by first converting it into RNA.