Electrochemical biosensors for pathogen detection

Some Enterobacteriaceae possess the ability to survive in low-moisture environments for extended periods of time. Many of the reported food-borne outbreaks associated with low-moisture foods involve Salmonella contamination. The control of Salmonella in low-moisture foods and their production environments represents a significant challenge for all food manufacturers. This review summarises the current state of knowledge with respect to Salmonella survival in intermediate- and low-moisture food matrices and their production environments. The mechanisms utilised by this bacterium to ensure their survival in these dry conditions remain to be fully elucidated, however in depth transcriptiomic data is now beginning to emerge regarding this observation. Earlier research work described the effect(s) that low-moisture can exert on the long-term persistence and heat tolerance of Salmonella, however, data are also now available highlighting the potential cross-tolerance to other stressors including commonly used microbicidal agents. Sources and potential control measures to reduce the risk of contamination will be explored. By extending our understanding of these geno- and phenotypes, we may be able to exploit them to improve food safety and protect public health.

/pdf/mechanisms-of-survival-responses-and-sources-of-salmonella-6pgxsusos1.pdf

Mechanisms of survival, responses and sources of Salmonella in low-moisture environments.

https://www.zora.uzh.ch/id/eprint/129861/1/ZORA129861.pdf

The response of foodborne pathogens to osmotic and desiccation stresses in the food chain.

Dried fruits, vegetables, herbs, and spices are produced in and sourced from many countries worldwide, but they have been increasingly reported to be involved in outbreaks and alerts due to the presence of foodborne pathogens such as Salmonella. These dried products are mainly produced by solar drying and conventional air drying, but a wide range of drying technologies are available. From a technological point of view the general trend is to optimize and standardize the drying process to ensure high-quality products to be offered. Drying technologies are mainly evaluated for their performance to reduce water activity at low energy cost while maintaining good sensorial quality of the dried product. However, as low water activity foods are increasingly recognized to support microbial survival and dried products are often consumed as they are, or are used as ingredients in many ready-to-eat foods, there is increasing attention to the microbiological quality and safety aspects of these products. This review presents traditional and emerging technologies to dry fruits, vegetables, herbs, and spices and discusses their potential to inactivate bacteria and viruses throughout the drying process. Overall, the microbial inactivation effect of the presented technologies has not yet been thoroughly assessed, even for traditional methods like solar drying, conventional air drying, or freeze-drying. Emerging technologies such as dielectric (assisted) drying and low-pressure superheated steam drying have been shown to reduce microbial populations; however, the number of studies is still low. Very few studies have focused on viral inactivation during drying processes.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/1541-4337.12224

Performance of Drying Technologies to Ensure Microbial Safety of Dried Fruits and Vegetables

https://static-curis.ku.dk/portal/files/123841276/review_published.pdf

Harry Beckers

Papers

Low-water activity foods: increased concern as vehicles of foodborne pathogens