Nitrate respiration in relation to facultative metabolism in enterobacteria.

We examined the properties of mutants of E. coli which are defective with respect to nitrate reductase activity. chlE::Mu cts and chlG::Mu cts mutants were all chlorate resistant, and the strains that we examined all synthesized nitrate reductase apoenzyme. We concluded that the chlE and chlG loci, like the chlA, chlB, and chlD loci, are involved in the synthesis of insertion of molybdenum cofactor. We identified at least four distinct phenotypic classes of chlC::Tn10 mutants, all of which were fully or partially sensitive to chlorate. Two of these classes may represent lesions in the structural genes for nitrate reductase subunits A and C. Two other classes may be altered in the regulation of the expression of nitrate reductase or other anaerobic enzymes. We propose the mnemonic nar for naming individual genes within the chlC locus.

Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.

I used a chlC-lac operon fusion to study regulatory mutations which affect nitrate reductase expression in Escherichia coli. A NarL- mutant apparently lacks a nitrate-specific positive regulatory component. Furthermore, an fnr (nirR) mutation prevented enzyme induction under any conditions. These data are consistent with a two-step, positive control model for nitrate reductase regulation.

Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12.

Genetic map of Salmonella typhimurium, edition VIII.

https://www.cell.com/cell/pdf/0092-8674(84)90245-9.pdf

Helen E. Fukumoto

Papers

Mutants of Salmonella typhimurium deffective in the reduction of trimethylamine oxide

Manual Salicylate-Hypochlorite Procedure for Determination of Ammonia in Kjeldahl Digests

Pancreatobiliary and biliary control of fecal β-glucuronidase activity in the rat