The idea of using a chromatographic adsorbent in the form of a thin layer fixed on an inert rigid support seems to have been suggested by Izmailov and Shraiber in 1938. Meinhard and Hall[1] in 1949 developed this notion of an ‘open column’, and in 1951 Kirchner, Miller, and Keller[2] reported the separation of terpenes on a ‘chromatostrip’, prepared by coating a small glass strip with an adsorbent mixed with starch or plaster of Paris, which acted as a binder. The strips were handled in the same way that paper is handled in paper chromatography, and indeed the original object of the thin-layer technique was to apply the methods of paper partition chromatography to an adsorption system.

Thin Layer Chromatography

Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance.

Chromatographically homogeneous egg lecithin, as determined by TLC on Silica Gel G, has been isolated from crude egg phosphatides by column chromatography on alumina through modification of existing, lengthy methods. The modified method involved application of crude egg phosphatides to a column of alumina in the proportion of 1 g phosphatide/25 g alumina, and elution of the lecithin fraction with the 2-component solvent system chloroform:methanol, 9:1 by vol. This method of purification separated lecithin from other choline and non-choline components of crude phosphatides, avoided overloading of the alumina column, and made unnecessary the need for a second chromatographic fractionation of partially purified lecithin on silicic acid, which is needed in existing methods of purification of lecithin. The use of fresh yolks permitted easier removal of pigment from the final product than was possible with commercially dried yolks. Phosphatides extracted from dried yolks were much more highly colored than were the phosphatides extracted from fresh yolks and the color presisted through chromatography on alumina. The fatty acid/phosphorus molar ratio of the purified lecithin was 2.00, which is the theoretical FA/P molar ratio of phosphatidylcholine; other materials with this ratio were not present.

Chromatographically homogeneous lecithin from egg phospholipids.

Esterification of Fatty Acids with Diazomethane on a Small Scale

Chromatographic separation, identification, and analysis of phosphatides

Technique
Thin-Layer Chromatography as an Analytical Tool


Qualitative Analysis


Quantitative Analysis





Thin-Layer Chromatography as a Preparative Tool

Thin-layer chromatography of lipids

Adsorption chromatography on thin layers of silicic acid or alumina provides a new and highly efficient analytical tool for the rapid separation of lipids according to classes of compounds.

Fractionation of fats, oils, and waxes on thin layers of silicic acid

Further work has been done on the application of thin-layer adsorption chromatography to the fractionation of complex lipid mixtures into classes.

Helmut K. Mangold

Papers

Thin-layer chromatography of lipids

Fractionation of fats, oils, and waxes on thin layers of silicic acid

Analysis of complex lipid mixtures by thin-layer chromatography and complementary methods

Reactions of Aliphatic Methanesulfonates. I. Syntheses of Long-Chain Glyceryl-(1) Ethers1

Extraction of seed oils with liquid and supercritical carbon dioxide