Heping Zhou

TL;DR: A statistical filter based on the correlation of data from “reverse‐labeled” experiment sets accurately predicted the agreement between the microarray measurements and enzyme‐linked immunosorbent assay measurements, showing that this parameter can serve to screen for antibodies that are functional on microarrays.

TL;DR: Replicate RCA measurements of multiple proteins from sets of 24 serum samples were highly reproducible and accurate, and RCA enabled reproducible measurements of distinct expression profiles from lower-abundance proteins that were not measurable using the other detection methods.

TL;DR: This study establishes reliable normalization for antibody microarray data, criteria for assessing normalization performance, and the capability of antibody microarrays for serum-protein profiling and multiparametric sample classification.

TL;DR: The use of microarrays of tumor‐derived proteins to profile the antibody repertoire in the sera of prostate cancer patients and controls suggests that microarray of fractionated proteins could be a powerful tool for tumor antigen discovery and cancer diagnosis.

TL;DR: This chapter describes methods for the production and use of antibody microarrays, divided into antibody handling and microarray production, (a) sample preparation, and (c) microarray use.