H
Hideki Aizaki
Researcher at National Institutes of Health
Publications - 120
Citations - 6246
Hideki Aizaki is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Hepatitis C virus & Virus. The author has an hindex of 42, co-authored 112 publications receiving 5692 citations. Previous affiliations of Hideki Aizaki include Jikei University School of Medicine & University of Southern California.
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Journal ArticleDOI
Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
Takahiro Masaki,Ryosuke Suzuki,Kyoko Murakami,Hideki Aizaki,Koji Ishii,Asako Murayama,Tomoko Date,Yoshiharu Matsuura,Tatsuo Miyamura,Takaji Wakita,Tetsuro Suzuki +10 more
TL;DR: It is demonstrated that NS5A is a prerequisite for HCV particle production as a result of its interaction with the viral capsid protein (core protein), and that the alanine substitutions in the serine cluster suppressed the association of the core protein with viral genome RNA, possibly resulting in the inhibition of nucleocapsid assembly.
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Interactions between viral nonstructural proteins and host protein hVAP-33 mediate the formation of hepatitis C virus RNA replication complex on lipid raft.
TL;DR: It is shown that a cellular vesicle membrane transport protein named hVAP-33, which binds to both NS5A and NS5B, plays a critical role in the formation of HCV replication complex, and is partially associated with the detergent-resistant membrane fraction.
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Hepatitis C Virus RNA Replication Occurs on a Detergent-Resistant Membrane That Cofractionates with Caveolin-2
TL;DR: This study labels de novo-synthesized viral RNA in situ with bromouridine triphosphate (BrUTP) in Huh7 cells expressing an HCV subgenomic replicon and suggests that HCV RNA synthesis occurs on a lipid raft membrane structure.
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Characterization of the hepatitis C virus RNA replication complex associated with lipid rafts.
TL;DR: Detergent-resistant membrane (DRM) fractions containing NS proteins and viral RNA were capable ofHCV RNA synthesis using the endogenous HCV RNA template, and suggested that the HCV RCs are protected within lipid rafts.
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Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors
Ikuo Shoji,Hideki Aizaki,Hideki Tani,Koji Ishii,Tsutomu Chiba,Izumu Saito,Tatsuo Miyamura,Yoshiharu Matsuura +7 more
TL;DR: The results suggest that the baculovirus vector is a good tool for gene delivery into various mammalian cells in order to study the function of foreign genes.