H
Hideo Hayashi
Researcher at University of Tsukuba
Publications - 69
Citations - 5804
Hideo Hayashi is an academic researcher from University of Tsukuba. The author has contributed to research in topics: Gene & Clostridium perfringens. The author has an hindex of 34, co-authored 68 publications receiving 5604 citations.
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Journal ArticleDOI
Sequence analysis of flanking regions of the pfoA gene of Clostridium perfringens: beta-galactosidase gene (pbg) is located in the 3'-flanking region.
TL;DR: The 3′‐flanking region of the perfringolysin O (theta‐toxin) gene (pfoA) of Clostridium perfringens was analyzed by chromosome walking and the pBG gene was subcloned into pBR322 and was successfully expressed in Escherichia coli, suggesting that the pbg gene codes for a β‐galactosidase of C. perfringen.
Journal ArticleDOI
Transcriptional analysis of the β-galactosidase gene (pbg) in Clostridium perfringens
TL;DR: The promoter structure of the pbg operon was characterized by many palindrome structures and direct repeats, which suggests that there might be some catabolite regulation of the expression of the PBG operon in C. perfringens.
Journal Article
Trichinella spiralis-specific monoclonal antibodies and affinity-purified antigen-based diagnosis.
Potjanee Srimanote,Wannaporn Ittiprasert,B. Sermsart,Urai Chaisri,Pakpimol Mahannop,Yuwaporn Sakolvaree,Pramuan Tapchaisri,Wanchai Maleewong,Hisao Kurazono,Hideo Hayashi,Wanpen Chaicumpa +10 more
TL;DR: Dot-blot ELISA is the method of choice for the rapid diagnosis of trichinellosis, particularly when more complex laboratory tests are unavailable, and is easier to perform, more rapid and less expensive than indirect ELISA.
Journal ArticleDOI
Staphylococcal Drp35 is the functional counterpart of the eukaryotic PONs
TL;DR: It is demonstrated that Drp35 is a lactonase that does not contribute directly to the resistance to the inducer antibiotics except for bacitracin.
Journal Article
Validation of salmonellosis and shigellosis diagnostic test kits at a provincial hospital in Thailand.
Sonjai K,Soisangwan R,Yuwaporn Sakolvaree,Hisao Kurazono,Manas Chongsa-nguan,Pramuan Tapchaisri,Yuvadee Mahakunkijcharoen,G.B. Nair,Hideo Hayashi,Wanpen Chaicumpa +9 more
TL;DR: The present study was designed to validate the accuracy of the test kits in comparison with the conventional bacterial culture method alone or with the combined results of the culture and the Western blot analysis (WB) for detecting the respective bacterial lipopolysacchharides (LPS) in specimens.