Hiroko Hama

TL;DR: Findings indicate that, first, PtdIns(4)P limitation is a major contributing factor to the secretory defect in sec14 cells; second, Sec14p function is coupled to the action of Pik1p, and; third, PTDIns( 4)P has an important role in the Golgi-to-plasma membrane stage of secretion.

TL;DR: It is reported that Arabidopsis plants grown in liquid media rapidly increase PtdIns(4,5)P(2) synthesis in response to treatment with sodium chloride, potassium chloride, and sorbitol, and this data suggest that when challenged with salinity and osmotic stress, terrestrial plants respond differently than algae, yeasts, and animal cells that accumulate different species of phosphoinositides.

TL;DR: It is proposed that activated-Vps21p interacts with its effector, Vac1p, which interacts with Vps45p to regulate the Golgi to endosome SNARE complex.

TL;DR: It is concluded that Vps9p is a novel guanine nucleotide exchange factor that is specific for Vps21p/Rab5 and may also possess unique regulatory functions required for vacuolar protein transport.

TL;DR: Catalytically inactive derivatives of these compartment-restricted Pik1 constructs indicated that PtdIns4P must be generated both in the nucleus and at the Golgi for normal cell function.