H
Hiroshi Shimada
Researcher at Hiroshima University
Publications - 79
Citations - 3925
Hiroshi Shimada is an academic researcher from Hiroshima University. The author has contributed to research in topics: Chloroplast & Arabidopsis. The author has an hindex of 30, co-authored 79 publications receiving 3621 citations. Previous affiliations of Hiroshi Shimada include Tokyo Institute of Technology.
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Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate.
Tohru Tsuchiya,Hiroyuki Ohta,Katsuya Okawa,Akihiro Iwamatsu,Hiroshi Shimada,Tatsuru Masuda,Ken-ichiro Takamiya +6 more
TL;DR: The Chlase cDNA isolated in the present study shared 37% identity with a function-unknown gene whose mRNA is inducible by coronatine and methyl jasmonate in Arabidopsis thaliana and expressed the gene products of AtCLH1 and of CaCLH in Escherichia coli, and they similarly exhibited Chlases activity.
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Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana
Koichiro Awai,Eric Maréchal,Maryse A. Block,Delphine Brun,Tatsuru Masuda,Hiroshi Shimada,Ken-ichiro Takamiya,Hiroyuki Ohta,Jacques Joyard +8 more
TL;DR: Type B isoforms with the enzymes of type A that are known to sit in the inner membrane of plastid envelope are compared to show that both types are not specialized isoforms for the prokaryotic and eukaryotic glycerolipid biosynthetic pathways.
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Monitoring of methyl jasmonate-responsive genes in Arabidopsis by cDNA macroarray: self-activation of jasmonic acid biosynthesis and crosstalk with other phytohormone signaling pathways.
Yuko Sasaki,Erika Asamizu,Daisuke Shibata,Yasukazu Nakamura,Takakazu Kaneko,Koichiro Awai,Masayuki Amagai,Chikara Kuwata,Taneaki Tsugane,Tatsuru Masuda,Hiroshi Shimada,Ken-ichiro Takamiya,Hiroyuki Ohta,Satoshi Tabata +13 more
TL;DR: The results suggest the existence of a positive feedback regulatory system for JA biosynthesis and the possibility of crosstalk between JA signaling and other signaling pathways.
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Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts
Norihiko Misawa,Hiroshi Shimada +1 more
TL;DR: It has been observed in the yeasts S. cerevisiae and C. utilis carrying the lycopene biosynthesis genes that ergosterol content is decreased by 10 and 35%, respectively, and it is likely that the carbon flux for the ergosterols biosynthesis has been partially directed from farnesyl pyrophosphate to a new pathway for the lyCopenoid biosynthesis.
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Increased Carotenoid Production by the Food Yeast Candida utilis through Metabolic Engineering of the Isoprenoid Pathway
TL;DR: Through metabolic engineering of the isoprenoid pathway, a sevenfold increase in the yield of lycopene has been achieved through modifications in related biochemical pathways can be utilized to enhance the production of commercially desirable compounds such as carotenoids.