KEGG(Kyoto Encyclopedia of Genes and Genomes)〔和文〕 (特集 ゲノム医学の現在と未来--基礎と臨床) -- (データベース)

The agriGO platform, which has been serving the scientific community for >10 years, specifically focuses on gene ontology (GO) enrichment analyses of plant and agricultural species. We continuously maintain and update the databases and accommodate the various requests of our global users. Here, we present our updated agriGO that has a largely expanded number of supporting species (394) and datatypes (865). In addition, a larger number of species have been classified into groups covering crops, vegetables, fish, birds and insects closely related to the agricultural community. We further improved the computational efficiency, including the batch analysis and P-value distribution (PVD), and the user-friendliness of the web pages. More visualization features were added to the platform, including SEACOMPARE (cross comparison of singular enrichment analysis), direct acyclic graph (DAG) and Scatter Plots, which can be merged by choosing any significant GO term. The updated platform agriGO v2.0 is now publicly accessible at http://systemsbiology.cau.edu.cn/agriGOv2/.

agriGO v2.0: a GO analysis toolkit for the agricultural community, 2017 update.

Cloning and characterization of the

Differentiation and secondary metabolism are correlated processes in fungi that respond to light. In
Aspergillus nidulans
, light inhibits sexual reproduction as well as secondary metabolism. We identified
the heterotrimeric
velvet
complex VelB/VeA/LaeA connecting light-responding developmental
regulation and control of secondary metabolism. VeA, which is primarily expressed in the dark,
physically interacts with VelB, which is expressed during sexual development. VeA bridges VelB to the
nuclear master regulator of secondary metabolism, LaeA. Deletion of either
velB
or
veA
results in
defects in both sexual fruiting-body formation and the production of secondary metabolites.

VelB/VeA/LaeA Complex CoordinatesLight Signal with Fungal Developmentand Secondary Metabolism

Molecular activities, biosynthesis and evolution of triterpenoid saponins.

The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas gene editing technology has opened a new era of genome interrogation and genome engineering because of its ease operation and high efficiency. An increasing number of plant species have been subjected to site-directed gene editing through this technology. However, the application of CRISPR-Cas technology to medicinal plants is still in the early stages. Here, we review the research history, structural characteristics, working mechanism and the latest derivatives of CRISPR-Cas technology, and discussed their application in medicinal plants for the first time. Furthermore, we creatively put forward the development direction of CRISPR technology applied to medicinal plant gene editing. The aim is to provide a reference for the application of this technology to genome functional studies, synthetic biology, genetic improvement, and germplasm innovation of medicinal plants. CRISPR-Cas is expected to revolutionize medicinal plant biotechnology in the near future. 

/pdf/crispr-cas-gene-editing-technology-and-its-application-3t433igy.pdf

CRISPR-Cas gene editing technology and its application prospect in medicinal plants

Mevalonate kinase (MVK) is a key enzyme in plant terpenoid biosynthetic pathway This study focused on cloning and analysis of Panax notoginseng (Burk) F H Chen MVK (PnMVK1) gene After searching the transcriptome dataset of P notoginseng, one unique sequence encoding MVK was discovered The primers were designed according to the transcript sequence of PnMVK1 from the P notoginseng transcriptome dataset And then, the open reading frame of PnMVK1 was cloned from P notoginseng by using RT-PCR strategy The physical and chemical properties, secondary structure and three-dimensional structure of the PnMVK1 protein were forecasted and analyzed, and its structure and function were predicted The cDNA (named as PnMVK1) contains a 1164 bp open reading frame and encodes a predicted protein of 387 amino acids The GenBank accession number for this gene is JQ957844 No transmembrane region and signal peptide were present in PnMVK1 The conserved domain of mevalonate kinase was present in PnMVK1 PnMVK1 was more abundant in P notoginseng root than other organisms This study cloned and analyzed PnMVK1 gene from P notoginseng for the first time The result will provide a foundation for exploring the mechanism of terpenoid biosynthesis in P notoginseng plants

Cloning and analysis of mevalonate kinase (PnMVK1) gene in Panax notoginseng

Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate.

Cloning and Bioinformatic Analysis of HMGS and HMGR Genes from Panax notoginseng

Transcription factor is one of the key factors in the regulation of gene expression at the transcriptional level. It plays an important role in plant growth, active components biosynthesis and response to environmental change. This paper summarized the structure and classification of bHLH transcription factors and elaborated the research progress of bHLH transcription factors which regulate the active components in plants, such as flavonoids, alkaloids, and terpenoids. In addition, the possibility of increasing the concentration of active substances by bHLH in medicinal plants was assessed. The paper emphasized great significance of model plants and multidisciplinary research fields including modern genomics, transcriptomics, metabolomics and bioinformatics, providing the contribution to improve the discovery and function characterization of bHLH transcription factors. Accelerating the research in the mechanism of bHLH transcription factors on the regulation of active components biosynthesis will promote the development of breeding and variety improvement of Chinese medicinal materials, also ease the pressure of resources exhaustion of traditional Chinese medicine home and abroad.

Hongmei Luo

Papers

CRISPR-Cas gene editing technology and its application prospect in medicinal plants

Cloning and analysis of mevalonate kinase (PnMVK1) gene in Panax notoginseng

Cloning and analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase genes HsHDR1 and HsHDR2 in Huperzia serrate.

Cloning and Bioinformatic Analysis of HMGS and HMGR Genes from Panax notoginseng

Research progress of the regulation on active compound biosynthesis by the bHLH transcription factors in plants