Compared with the single-marker detection scheme, the detection of multiple targets in the complex cell and biological environment can obtain more reliable detection results. Herein, we detected miRNA-21 and APE1 in two modes, AND and OR, respectively, based on gold nanoflares and simple logic components. In both modes, DNAzyme and APE1 can get rich fluorescence recovery results by breaking the DNA strands from the gold nanorods (AuNRs) and unquenching under different conditions. In vivo and in vitro experiments suggest that both nanoflares exhibit excellent biocompatibility and make efficient and sensitive judgments on the two targets. This strategy emphasizes the reuse nature of enzymes, and a small amount of target can generate a large amount of fluorescent signal in the logic device, which greatly reduces the detection limit when monitoring low-abundance targets. Since the short-stranded DNA component of the detection device is simple in composition and easy to program its probe sequence, it can be expanded into a detection system for the detection of other sets of related markers, which increases its potential for clinical application.

Smart Programmable Scalable Dual-Mode Diagnostic Logic Nanoflare Strategy for Dual-Tumor Marker Detection.

Organophosphorus chemical security from a peaceful perspective: sustainable practices in its synthesis, decontamination and detection

The invention relates to a preparation method of triphenyl phosphate. The preparation method comprises the following steps of: (1), adding a catalyst into molten phenol, putting a mixture into a reaction kettle, stirring the mixture until the temperature is reduced to be below 50 DEG C, stopping stirring and mixing phosphorus oxychloride; (2), performing constant pressure acid drainage and negative pressure acid drainage under a stirring condition to ensure that an acid value is less than or equal to 6mg KOH/g, and neutralizing and exhausting to obtain qualified coarse ester; and (3) raising the temperature of the qualified coarse ester to perform pressure reduction, distillation and separation and collecting a finished product; performing alkali washing and water washing, sampling and analyzing the finished product to ensure that the acid value is less than or equal to 0.05mg KOH/g, dehydrating and purifying, and sampling and analyzing the finished product to ensure that the acid value is less than or equal to 0.05mg KOH/g; and filtering to obtain triphenyl phosphate. The preparation method is easy to operate, mild in condition, short in reaction time, low in energy consumption, low in corrosion, low in pollution, and high in yield, and the product is pure, and the yield is up to more than 88 percent.

Preparation method of triphenyl phosphate

On-Water and Efficient Ullmann-Type O-Arylation Cross Coupling Reaction of Phenols and Aryl Tosylates in the Presence of Fe3O4@Starch-Au as Nanocatalyst

The invention discloses a preparation method of aluminium hypophosphite. The preparation method comprises the following steps of: a, preparing a sodium hypophosphite solution; b, preparing an aluminium nitrate solution; and c, uniformly mixing the sodium hypophosphite solution with the aluminium nitrate solution, regulating the pH (potential of Hydrogen) to 2-6, heating and reacting to form the target product aluminium hypophosphite. The method disclosed by the invention has the advantages that the reaction is carried out through directly taking water as solvent and the temperature is lower, so that the reaction is easy to control; the particle size of the obtained aluminium hypophosphite is less than 50 micrometers, the aluminium hypophosphite is white powder and no extra color processing is needed, so that the purity is higher and the yield of the obtained target product is 92-99%; the conventional synthetic process route is simplified and no toxic substance is produced, so that the preparation method is friendly to environment and low in cost; and high-efficiency fire retardant and combustion improver are obtained in the same reaction, so that the resource utilization rate is high and better industrial prospect is provided.

Preparation method of aluminium hypophosphite

High quality synthesis of Rh nanocubes and their application in hydrazine hydrate oxidation assisted water splitting

The invention relates to a structure and preparation method of a furanose cyclic phosphoric ester fire retardant. The preparation method is simple in technology, the raw materials are low in cost, theenvironmental pollution is low, and the prepared cyclic phosphoric ester fire retardant is prominent in fire retardance and industrialization is easy to realize.

Structure and preparation method of furanose cyclic phosphoric ester fire retardant

The invention discloses a method for synthesizing tetrabromobisphenol A bis(2,3-dibromopropyl) ether in a water phase. The method comprises the steps that a surfactant and tetrabromobisphenol A diallyl ether are mixed in the water phase to prepare emulsion; bromine is dropwise added, and the tetrabromobisphenol A diallyl ether and the bromine are subjected to an addition reaction to obtain the tetrabromobisphenol A bis(2,3-dibromopropyl) ether; after the reaction is finished, cooling, filtering, washing and drying are performed, and then the white solid product is obtained. According to the method, water is taken as a reaction medium, organic solvent is not used, and the problems generated due to the fact that the organic solvent is used are solved; the crystallization and sedimentation process of the tetrabromobisphenol A bis(2,3-dibromopropyl) ether is avoided, aftertreatment is simple, an existing technology route is greatly simplified, and the cost is effectively lowered; the method is friendly to environment, the product is white in color, good in quality and high in yield, the technology is simple, and the method is suitable for industrialized application; the yield of the obtained product can reach 93.1%, and the purity of the obtained product can reach 95.5%.

Method for synthesizing tetrabromobisphenol A bis(2,3-dibromopropyl) ether in water phase

The invention discloses applications of a halogen-free cyclic pyrophosphate in preparation of fire-retardant rigid polyurethane foamed plastic. According to the applications, a polyhydric compound, afoam stabilizing agent, a foaming agent, a catalyst, an isocyanate, and a fire retardant are taken as raw materials for compounding processing so as to obtain the fire-retardant rigid polyurethane foamed plastic. The fire retardant is a halogen-free cyclic pyrophosphate which is high in phosphorus content, and possesses a cyclic structure, so that the fire retardant is excellent in fire resistanceand heat stability, and the high efficiency environment-friendly fire-retardant rigid polyurethane foamed plastic can be prepared. The fire retardant halogen-free cyclic pyrophosphate is high in flame resistance efficiency, and low in using amount; the cost is reduced; the compatibility of the halogen-free cyclic pyrophosphate with a base material is excellent; migration or bleedout is not easilycaused. The prepared high efficiency environment-friendly fire-retardant rigid polyurethane foamed plastic are abundant in raw material sources, and low in cost; the preparation method is simple; industrial conditions are easy to control; and the preparation method is suitable for industrialized production.

Applications of halogen-free cyclic pyrophosphate in preparation of fire-retardant rigid polyurethane foamed plastic

The invention discloses a method for preparing cyclic phosphate or cyclic phosphite. The method comprises the steps: (1) mixing a substance A with an alcoholic compound so as to obtain a mixture, andthen, heating the temperature of the mixture to 110 DEG C to 150 DEG C, wherein the substance A is triphosphate or triphosphite; (2) adding an organic amine catalyst into the mixture, and carrying outan ester exchange reaction for 5 to 9 hours so as to obtain a prepared substance, wherein the prepared substance is cyclic phosphate when the substance A is triphosphate, and the prepared substance is cyclic phosphite when the substance A is triphosphite; (3) carrying out distillation to remove impurities. According to the method, the cyclic phosphate or cyclic phosphite is synthesized in one step by adopting an ester exchange process by adopting organic amine as the catalyst, a solvent is not used, aftertreatment is not required, byproducts can be recycled, and the process route is simple.

Huang Xiaodong

Papers

High quality synthesis of Rh nanocubes and their application in hydrazine hydrate oxidation assisted water splitting

Structure and preparation method of furanose cyclic phosphoric ester fire retardant

Method for synthesizing tetrabromobisphenol A bis(2,3-dibromopropyl) ether in water phase

Applications of halogen-free cyclic pyrophosphate in preparation of fire-retardant rigid polyurethane foamed plastic

Method for preparing cyclic phosphate or cyclic phosphite