Ian M. Derrington

TL;DR: The ability to resolve changes in current that correspond to a known DNA sequence is demonstrated by combining the high sensitivity of a mutated form of the protein pore Mycobacterium smegmatis porin A with phi29 DNA polymerase (DNAP), which controls the rate of DNA translocation through the pore.

TL;DR: It is demonstrated that the ionic current through the engineered Mycobacterium smegmatis porin A, MspA, has the ability to distinguish all four DNA nucleotides and resolve single-nucleotides in single- Stranded DNA when double-stranded DNA temporarily holds the nucleotide in the pore constriction.

TL;DR: This work designs and constructed an MspA mutant capable of electronically detecting and characterizing single molecules of ssDNA as they are electrophoretically driven through the pore, and highlights its potential as an engineerable platform for single-molecule detection and characterization applications.

TL;DR: This work builds on recent progress with respect to nanopore resolution and DNA control to interpret the procession of ion current levels observed during the translocation of DNA through the pore MspA, and shows nanopore sequencing reads of phi X 174 up to 4,500 bases in length, which can be unambiguously aligned to the phiX 174 reference genome.

TL;DR: Pulling single-stranded DNA molecules through the biological pore MspA is found to reveal the methylation sites with high confidence and comparing current levels generated with DNA containing methylated CpG sites to current levels obtained with unmethylated copies of the DNA reveals the precise location of methylated PG sites.