Showing papers by "Ivone Antônia de Souza published in 2016"

Toxicological evaluation of essential oil from the leaves of Croton tetradenius (Euphorbiaceae) on Aedes aegypti and Mus musculus

Abstract: For control of Aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative Herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of Croton tetradenius on Ae aegypti larvae and adults We also evaluated the acute toxicity in Mus musculus The essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection Female mice were used for assessing toxicity according to the Organization for Economic Cooperation and Development's Test Guideline 423/2001 Doses administered to mice orally and intraperitoneally were 5, 50, 300, and 2000 mg kg(-1) There was a greater toxic effect on larvae (LC50 = 0152 mg mL(-1) and LC90 = 0297 mg mL(-1)) and on adults (LC50 = 1842 mg mL(-1) and LC90 = 3156 mg mL(-1)) of Ae aegypti after 24 h of exposure, when compared to other periods of exposure Chemical analysis revealed 26 components, with camphor (2549 %) as the major component The acute toxicity via the intraperitoneal route identified an LD50 = 200 mg kg(-1) and by the oral route an LD50 = 500 mg kg(-1) Thus, the essential oil of C tetradenius presents insecticidal potential for Ae aegypti and has high safety threshold at the concentrations evaluated in this study

Phytochemical Screening and Acute Toxicity of Aqueous Extract of Leaves of Conocarpus erectus Linnaeus in Swiss Albino Mice

Abstract: Mangroves represent areas of high biological productivity and it is a region rich in bioactive substances used in medicine production. Conocarpus erectus (Combretaceae) known as button mangrove is one of the species found in mangroves and it is used in folk medicine in the treatment of anemia, catarrh, conjunctivitis, diabetes, diarrhea, fever, gonorrhea, headache, hemorrhage, orchitis, rash, bumps and syphilis. The present study aimed to investigate the acute toxicity of aqueous extract of leaves of C. erectus in Swiss albino mice. The plant material was collected in Vila Velha mangroves, located in Itamaraca (PE). The material was subjected to a phytochemical screening where extractive protocols to identify majority molecules present in leaves were used. The evaluation of acute toxicity of aqueous extract of C. erectus followed the model of Acute Toxicity Class based on OECD 423 Guideline, 2001. The majority molecules were identified: flavonoids, tannins and saponins. The LD50 was estimated at 2,000 mg/kg bw. Therefore, the aqueous extract showed low acute toxicity classified in category 5.

Antinociceptive and healing activity of the methanolic and hydroethanolic extracts of Caulerpa taxifolia

Abstract: This study aimed to evaluate the healing and analgesic action of methanolic extract and hydroalcoholic of C. taxifolia, and check the percentage of soluble proteins available in the extract of this vegetable. The collection of C. taxifolia occurred on the beach of Barra do Sirinhaem - PE, processed for testing. The animals used in the test were Swiss mice, and Mus musculus of species. For healing activity and histopathological analysis, albino rats Rattus norvegicus species, linnhagem Wistar were used. The protein content of the crude extract of C. Taxifolia has not yet been determined. The data revealed that the average displaying writhing in animal control group was 13.86. The standard group was 7.83; the group treated with hydroethanolic extract of C. taxifolia at a dose of 50 mg/kg was 8.83 and those treated with 100 mg/kg was 6.5. In the groups treated with the methanol extract of 100 mg/kg, the average value of writhing was 7.67, while 50 mg/kg resulted in 9.26 average value. It was shown that hydroethanolic and methanol extract led to early healing ofwound with epithelialized keratinized tissue and re-epithelialization; involving the restructuring of the skin appendages, during treatment and in the absence of crust. Key words: Healing, seaweed, protein, repair.