Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.

The use of biocatalysis for industrial synthetic chemistry is on the verge of significant growth. Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds as well as water-soluble compounds can be modified selectively and efficiently with enzymes and biocatalytically active cells. As the use of biocatalysis for industrial chemical synthesis becomes easier, several chemical companies have begun to increase significantly the number and sophistication of the biocatalytic processes used in their synthesis operations.

Industrial biocatalysis today and tomorrow

Mechanisms of membrane toxicity of hydrocarbons.

Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters

We have designed a modular protein tagging system that allows different functionalities to be linked onto a single genetic fusion, either in solution, in living cells, or in chemically fixed cells. The protein tag (HaloTag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (HaloTag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Covalent bond formation between the protein tag and the chloroalkane linker is highly specific, occurs rapidly under physiological conditions, and is essentially irreversible. We demonstrate the utility of this system for cellular imaging and protein immobilization by analyzing multiple molecular processes associated with NF-κB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein−protein and protein−DNA complexes.

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli.

The growth of Pseudomonas oleovorans on n-octane was characterized by the formation of intracellular structures. These inclusions were isolated and characterized. Morphologically, they resembled the poly-beta-hydroxybutyrate granules found in Bacillus cereus, as shown by freeze-fracture electron microscopy. The elemental analysis of isolated granules showed, however, that they do not contain poly-beta-hydroxybutyric acid. Instead, the analysis was consistent with a C8 polyester, which interpretation was supported by the fatty acid analysis of hydrolyzed granules. From the evidence presented here, we conclude that P. oleovorans forms poly-beta-hydroxyoctanoate granules when grown on n-octane.

Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane.

The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of escherichia coli

https://pure.rug.nl/ws/files/3623789/2004ChemBiolvLoo.pdf

Directed Evolution of Epoxide Hydrolase from A. radiobacter toward Higher Enantioselectivity by Error-Prone PCR and DNA Shuffling

https://pure.rug.nl/ws/files/14842526/1995JBiolChemPries.pdf

Jaap Kingma

Papers

An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli.

Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane.

The effect of toluene on the structure and permeability of the outer and cytoplasmic membranes of escherichia coli

Directed Evolution of Epoxide Hydrolase from A. radiobacter toward Higher Enantioselectivity by Error-Prone PCR and DNA Shuffling

Histidine 289 Is Essential for Hydrolysis of the Alkyl-enzyme Intermediate of Haloalkane Dehalogenase