J
Jacques P. M. Schellens
Researcher at University of Amsterdam
Publications - 19
Citations - 1123
Jacques P. M. Schellens is an academic researcher from University of Amsterdam. The author has contributed to research in topics: Acid phosphatase & Substrate (chemistry). The author has an hindex of 10, co-authored 19 publications receiving 1086 citations.
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Journal ArticleDOI
The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes
E. F. C. Blommaart,Ulrike Krause,Jacques P. M. Schellens,Heleen Vreeling-Sindelárová,Alfred J. Meijer +4 more
TL;DR: 3-Methyladenine, which is commonly used as a specific inhibitor of autophagic sequestration, was an inhibitor of phosphatidylinositol 3-kinase, thus providing a target for its action.
Journal ArticleDOI
Hepatic autophagy and intracellular ATP. A morphometric study.
TL;DR: The data indicate that changes in intracellular ATP primarily affect early steps in the autophagic/proteolytic pathway, and shows that the rate of overall proteolysis via macroautophagy decreases with decreasing ATP concentration.
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Ultrastructural localization of xanthine oxidase activity in the digestive tract of the rat
R. J. M. Van Den Munckhof,H. Vreeling-Sindelarova,Jacques P. M. Schellens,C. J. F. Van Noorden,Wilma M. Frederiks +4 more
TL;DR: The intracellular localization of xanthine oxidase activity in the cytoplasm of epithelial cells as well as the extracellular localization suggest that the enzyme plays a role in the lumen of the digestive tract, for instance in the defence against microorganisms.
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The use of unfixed cryostat sections for electron microscopic study of D-amino acid oxidase activity in rat liver.
Jacques P. M. Schellens,Wilma M. Frederiks,C. J. F. Van Noorden,H. Vreeling-Sindelarova,F. Marx,P. J. Mcmillan +5 more
TL;DR: It is concluded that the semipermeable membrane technique opens new perspectives for localization of enzyme activities at the ultrastructural level without prior tissue fixation, thus enabling localization of the activity of soluble and/or labile enzymes.
Journal ArticleDOI
Localization of uric acid oxidase activity in core and matrix of peroxisomes as detected in unfixed cryostat sections of rat liver.
R. J. M. Van Den Munckhof,H. Vreeling-Sindelarova,Jacques P. M. Schellens,Wilma M. Frederiks +3 more
TL;DR: It is concluded that, because of the use of unfixed tissue, UAOX is not inactivated, which results in localization of UAOX activity not only in the core ofperoxisomes but also in the peroxisomal matrix.