James A. Gagnon

TL;DR: An online tool, CHOPCHOP, that uses efficient sequence alignment algorithms to minimize search times, and rigorously predicts off-target binding of single-guide RNAs (sgRNAs) and TALENs, making it a valuable tool for genome engineering.

TL;DR: Improved methods and detailed protocols make Cas9-mediated mutagenesis an attractive approach for labs of all sizes and increase rates of mutagenisation by implementing several novel approaches.

TL;DR: This major update of CHOPCHOP introduces tools for the next generation of CRISPR advances, including Cpf1 and Cas9 nickases, and provides support for custom length sgRNAs and evaluates the sequence composition of the whole sgRNA and its surrounding region using models compiled from multiple large-scale studies.

TL;DR: It is shown that combinatorial, cumulative genome editing of a compact barcode can be used to record lineage information in multicellular systems and that rich, systematically generated maps of organismal development will advance the understanding of development in both healthy and disease states.

TL;DR: Toddler is an essential, short, conserved embryonic signal that promotes cell migration during zebrafish gastrulation and helps explain previous genetic studies that found a broader requirement for APJ/Apelin receptors than for Apelin, and may be one of several uncharacterized embryonic signals.